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The use of fluorescently-tagged apoptolidins in cellular uptake and response studies

Katherine M Chong1,2, Nalin Leelatian1,3, Sean M Deguire1,2

  • 1Vanderbilt Institute of Chemical Biology, Vanderbilt University Medical Center, Nashville, TN, USA.

The Journal of Antibiotics
|March 10, 2016
PubMed

Insights

Apoptolidins, potent anti-cancer compounds, are selectively absorbed by cancer cells but not healthy blood cells. Fluorescently tagged apoptolidins aid in monitoring cellular uptake and response in cancer research.

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Pharmacology

Background:

  • Apoptolidins are glycomacrolide microbial metabolites.
  • They exhibit selective cytotoxicity against tumor cells.

Purpose of the Study:

  • To demonstrate selective uptake of fluorescently tagged apoptolidins in cancer cells.
  • To showcase the utility of these compounds in monitoring cellular response.

Main Methods:

  • Utilizing fluorescently tagged active apoptolidin derivatives.
  • Employing fluorescent flow cytometry to monitor cellular uptake and response.

Main Results:

  • Selective uptake of four tagged glycomacrolides in cancer cells over healthy human blood cells was observed.
  • Five fluorescently tagged glycomacrolides were utilized to monitor cellular uptake and response.

Conclusions:

  • Fluorescently tagged apoptolidins show selective uptake in cancer cells.
  • These tagged compounds are valuable tools for monitoring cellular processes in cancer research.

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