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Tissue-specific miRNA Expression Profiling in Mouse Heart Sections Using In Situ Hybridization
Published on: September 15, 2018
Bioinformatic and Expression Analysis of Ventricular Septal Defect-associated Long Non-coding RNA TUC40-
Hui-juan Li1, Li Jiang1, Zhang-bin Yu2
1Department of Pediatrics,Zhongda Hospital,Southeast University,Nanjing 210009,China.
Objective:
To explore the potential role of TUC40- in human and mouse embryonic heart development.
Methods:
Bioinformatics databases including NCBI,UCSC,and Uniprot and software including Clustal,DNAMAN,and MEGA 6 were used to collect information of TUC40- and uc.40-. The expression profile at key time points of heart development was investigated by strand-specific quantitative real time polymerase chain reaction.
Results:
Uc.40- was conservative in sequence, genomic location, and transcription factor binding sites across human and mouse. Pbx1/TUC40- showed negative trend during embryonic mouse heart maturation.
Conclusions:
Various levels of conservation of uc.40- suggests similar functions of TUC40- in these two species. TUC40- may play its roles in human and mouse embryonic heart development by regulating Pbx1.
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