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Area of Science:

  • Biomaterials Science
  • Polymer Chemistry
  • Drug Delivery Systems

Background:

  • Layer-by-layer (LbL) structures are promising for biomedical applications.
  • Understanding the degradation of LbL materials is crucial for their clinical translation.
  • Enzymatic degradation offers a tunable approach for LbL material breakdown.

Purpose of the Study:

  • To investigate the enzymatic degradation of chitosan/hyaluronic acid LbL structures.
  • To evaluate the impact of hyaluronidase on LbL films, membranes, and microcapsules.
  • To explore enzyme-mediated controlled release of active agents from LbL devices.

Main Methods:

  • Fabrication of chitosan/hyaluronic acid ultrathin films, freestanding membranes, and microcapsules.
  • Enzymatic degradation studies using varying concentrations of hyaluronidase.
  • Analysis of film thickness, topography, and membrane weight loss over time.
  • Encapsulation of FITC-BSA and hyaluronidase within microcapsules to assess drug release kinetics.

Main Results:

  • Hyaluronidase concentration-dependently reduced ultrathin film thickness and altered topography.
  • Freestanding membranes showed complete degradation within 120 hours in the presence of hyaluronidase.
  • Microcapsules co-loaded with hyaluronidase and FITC-BSA exhibited a fourfold increase in protein release compared to controls.

Conclusions:

  • Enzymatic degradation provides a tunable mechanism for controlling LbL structure breakdown.
  • Embedded enzymes can significantly enhance the release of co-encapsulated active agents.
  • This approach holds potential for controlled drug delivery in biomedical applications.