Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Edoxaban for atrial fibrillation in patients with atherosclerotic disease in daily clinical practice.

Journal of thrombosis and thrombolysis·2026
Same author

Serotype distribution and coverage by pneumococcal vaccines in hospitalised adults with pneumococcal pneumonia in Germany.

ERJ open research·2026
Same author

Mittag-Leffler expansion of the optical scattering matrix via rescaled scattering channels.

Optics letters·2026
Same author

Health disparities in the burden of pneumococcal disease in US adults.

Pneumonia (Nathan Qld.)·2026
Same author

The SureFast® Listeria 3plex ONE Kit and the SureFast® PREP Bacteria Kit in a Variety of Foods and Environmental Samples: Multi-Laboratory Validation, First Action 2025.04.

Journal of AOAC International·2026
Same author

Risk factors affecting the outcome of upper cervical spine injuries in the elderly - role of operative vs. conservative treatment.

European journal of trauma and emergency surgery : official publication of the European Trauma Society·2026

Related Experiment Video

Updated: Mar 24, 2026

Rapid Isolation of Human Breast Milk-Derived Extracellular Vesicles
04:35

Rapid Isolation of Human Breast Milk-Derived Extracellular Vesicles

Published on: November 14, 2025

533

Validation of the RIDASCREEN(®)FAST Milk Kit.

Thomas Weiss1, Markus Lacorn, Jonathan Flannery

  • 1R-Biopharm AG, An der neuen Bergstrasse 17, D-64297 Darmstadt, Germany.

Journal of AOAC International
|March 10, 2016
PubMed
Summary

The RIDASCREEN(®)FAST Milk test accurately quantifies milk proteins in food within 40 minutes. This enzyme-linked immunosorbent assay (ELISA) demonstrates high sensitivity and specificity for casein and β-lactoglobulin detection.

More Related Videos

Milk Collection in the Rat Using Capillary Tubes and Estimation of Milk Fat Content by Creamatocrit
07:38

Milk Collection in the Rat Using Capillary Tubes and Estimation of Milk Fat Content by Creamatocrit

Published on: December 16, 2015

13.3K
Individualized Reconstitution of Human Milk Microbiota: A Feasible Approach in Real-World Settings
04:16

Individualized Reconstitution of Human Milk Microbiota: A Feasible Approach in Real-World Settings

Published on: February 7, 2025

992

Related Experiment Videos

Last Updated: Mar 24, 2026

Rapid Isolation of Human Breast Milk-Derived Extracellular Vesicles
04:35

Rapid Isolation of Human Breast Milk-Derived Extracellular Vesicles

Published on: November 14, 2025

533
Milk Collection in the Rat Using Capillary Tubes and Estimation of Milk Fat Content by Creamatocrit
07:38

Milk Collection in the Rat Using Capillary Tubes and Estimation of Milk Fat Content by Creamatocrit

Published on: December 16, 2015

13.3K
Individualized Reconstitution of Human Milk Microbiota: A Feasible Approach in Real-World Settings
04:16

Individualized Reconstitution of Human Milk Microbiota: A Feasible Approach in Real-World Settings

Published on: February 7, 2025

992

Area of Science:

  • Food Science
  • Analytical Chemistry
  • Immunology

Background:

  • Accurate detection of milk proteins is crucial for food safety and allergen management.
  • Existing methods for milk protein quantification can be time-consuming and complex.
  • The need for rapid, reliable assays for milk protein analysis in diverse food matrices.

Purpose of the Study:

  • To evaluate the analytical performance of the RIDASCREEN(®)FAST Milk test for quantifying milk proteins.
  • To validate the assay's accuracy, sensitivity, specificity, and robustness across various food products.
  • To confirm the manufacturer's claims regarding the test's performance characteristics.

Main Methods:

  • Sandwich ELISA utilizing specific antibodies against casein and β-lactoglobulin.
  • Sample extraction followed by rapid analysis (under 40 minutes).
  • Validation across multiple food types (cookies, infant formula, chocolate dessert, ice cream, sausages) including incurred samples.

Main Results:

  • Assay demonstrated high sensitivity with a limit of detection (LOD) below 1 mg/kg and a limit of quantification (LOQ) of 2.5 mg/kg.
  • Recoveries for spiked samples were predominantly within the 80-120% range, with excellent lot-to-lot consistency.
  • No significant cross-reactivity was observed, and ruggedness testing confirmed stability against variations in assay parameters.

Conclusions:

  • The RIDASCREEN(®)FAST Milk test is a reliable and rapid method for milk protein quantification in various food products.
  • The assay exhibits excellent analytical performance, meeting manufacturer claims and demonstrating suitability for routine food analysis.
  • This ELISA offers a valuable tool for ensuring food safety and compliance with allergen labeling regulations.