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Related Concept Videos

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Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a  complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
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Related Experiment Video

Updated: Mar 24, 2026

Author Spotlight: Semi-Automated Isolation of the Stromal Vascular Fraction from Murine White Adipose Tissue Using a Tissue Dissociator
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Forkhead box A3 mediates glucocorticoid receptor function in adipose tissue.

Xinran Ma1, Lingyan Xu1, Elisabetta Mueller2

  • 1Genetics of Development and Disease Branch, National Institute of Diabetes and Digestive and Kidney Disease, National Institutes of Health, Bethesda, MD 20892.

Proceedings of the National Academy of Sciences of the United States of America
|March 10, 2016
PubMed
Summary

Glucocorticoids (GCs) cause fat accumulation, but the forkhead box protein A3 (Foxa3) mediates this effect. Targeting Foxa3 may prevent GC-induced obesity and related metabolic disorders.

Keywords:
Foxa3GR signalingadipose tissuedexamethasonefat expansion

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Area of Science:

  • Endocrinology
  • Molecular Biology
  • Metabolism

Background:

  • Glucocorticoids (GCs) are potent anti-inflammatory drugs with significant side effects, including obesity.
  • The forkhead box protein A3 (Foxa3) is implicated in regulating fat depot expansion and energy expenditure.
  • Mechanisms controlling Foxa3 expression in adipose tissue remain largely unknown.

Purpose of the Study:

  • To investigate the link between GCs and Foxa3 expression in adipose tissue.
  • To determine if Foxa3 mediates GC-induced adipose tissue expansion.

Main Methods:

  • Computational prediction analysis to identify potential regulatory interactions.
  • Molecular studies in preadipocytes, adipocytes, and adipose tissues.
  • In vivo studies using dexamethasone treatment and Foxa3 ablation in mice.

Main Results:

  • Foxa3 is directly regulated by the glucocorticoid receptor (GR) in various fat cells and tissues.
  • Foxa3 facilitates GR binding to target gene promoters in adipose tissue.
  • Foxa3 ablation specifically protected mice against dexamethasone-induced fat accretion, but not other GC side effects.

Conclusions:

  • Foxa3 is a direct target of GC action in adipose tissue.
  • Foxa3 acts as a mediator of GC-induced side effects in fat tissue.
  • Targeting Foxa3 may offer a strategy to mitigate GC-induced obesity.