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Published on: December 19, 2020
Immunological evaluation of the entirely carbohydrate-based Thomsen-Friedenreich - PS B conjugate
Kevin R Trabbic1, Jean-Paul Bourgault, Mengchao Shi
1Department of Chemistry and Biochemistry, University of Toledo, Wolfe Hall, Room 2232B, 2801 W. Bancroft Street, Toledo, OH 43606-3390, USA. Peter.Andreana@utoledo.edu.
Abstract:
PS B, a naturally occurring CD4(+) T-cell simulating zwitterionic polysaccharide from Bacteroides fragilis ATCC 25285/NCTC 9343, was conjugated with aminooxy Thomsen Friedenreich (TF or T) [α-d-Gal-(1,3)-β-d-GalNAc-ONH2] tumor antigen. Immunization in Jax C57BL/6, followed by ELISA revealed IgM and IgG antibody TF specificity. FACS data noted preferential binding to TF-laced MCF-7 cells but not to HCT-116 cells.
