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Updated: Mar 24, 2026
The 6-hydroxydopamine Rat Model of Parkinson's Disease
Published on: October 27, 2021
Effect of adenosine A2A receptor antagonists on motor disorders induced by 6-hydroxydopamine in rat
Siamak Reyhani-Rad1, Javad Mahmoudi2
1Department of Laboratory Sciences, Islamic Azad University, Marand, Iran.
Purpose:
To investigate the role of adenosine A2A receptors on 6-OHDA-induced motor disorder in rat.
Methods:
In order to induce experimental model of Parkinson's disease, 6-hydoxydopamine (8 μg/rat) was injected unilaterally into the SNc. After three weeks as a recovery period, 6-OHDA-induced bradykinesia and balance disturbances were assessed by using beam traversal test 10, 30 and 60 minutes after intraperitoneal injections of the drugs (caffeine, SCH58261).
Results:
The results showed that 6-OHDA (8 μg/rat, Intra-SNc) induced motor disorders of Parkinson's disease and increased elapsed time in the beam test (p<0.001). Injection of caffeine (30 mg/kg, i.p.) and SCH58261 (2 mg/kg, i.p.) attenuated elapsed time on beam (p<0.01 and p<0.001). We showed that acute administration of caffeine and SCH 58261 can improve the 6-OHDA-induced bradykinesia and motor disturbance.
Conclusion:
Adenosine A2AR antagonists improve 6-OHDA-motor deficit and this effect seems to be mediated by the inhibition of A2A presynaptic receptors in substantia nigra pars compacta.
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