Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Metabolism of Chemolithotrophs01:15

Metabolism of Chemolithotrophs

1.2K
Chemolithotrophs are microorganisms that obtain energy by oxidizing inorganic molecules such as hydrogen gas (H₂), ammonia (NH₃), reduced sulfur compounds (H₂S, S²⁻), and ferrous iron (Fe²⁺). Unlike heterotrophic organisms that rely on organic carbon, chemolithotrophs transfer electrons from these inorganic donors to the electron transport chain (ETC), generating a proton motive force (PMF) that drives ATP synthesis through oxidative phosphorylation.
1.2K
Bioremediation00:46

Bioremediation

22.8K
Bioremediation is the use of prokaryotes, fungi, or plants to remove pollutants from the environment. This process has been used to remove harmful toxins in groundwater as a byproduct of agricultural run-off and also to clean up oil spills.
22.8K
Microbial Bioremediation of Plastics01:28

Microbial Bioremediation of Plastics

30
Polyethylene terephthalate (PET) is a synthetic polymer widely utilized in the packaging industry, particularly for bottles and containers. Due to its chemical stability and durability, PET accumulates in the environment, contributing significantly to plastic pollution. It comprises repeating units of terephthalic acid and ethylene glycol, resulting in a semi-crystalline structure that is resistant to natural degradation processes.A notable breakthrough in plastic biodegradation came with the...
30
Inorganic Nitrogen Assimilation01:22

Inorganic Nitrogen Assimilation

787
Nitrogen is an essential element in biological systems, forming a crucial component of proteins, nucleic acids, and other cellular constituents. Many bacteria and archaea acquire nitrogen in the form of nitrate (NO₃⁻) or ammonia (NH₃), which are then assimilated into biomolecules through specific enzymatic pathways.Assimilatory Nitrate ReductionWhen nitrate enters the cell, it undergoes a two-step reduction process known as assimilatory nitrate reduction. Initially, the enzyme...
787
Environmental Applications of Microorganisms01:30

Environmental Applications of Microorganisms

1.4K
Microorganisms play a pivotal role in maintaining ecosystem balance by recycling essential elements such as carbon, nitrogen, and phosphorus, as well as supporting processes like bioremediation, wastewater treatment, and biofuel production.Microbes in Elemental CyclesIn the carbon cycle, microorganisms decompose organic matter, releasing carbon dioxide via aerobic respiration. This carbon dioxide is subsequently used by photosynthetic organisms to synthesize organic compounds, closing the...
1.4K
Carbon-dioxide Fixation01:28

Carbon-dioxide Fixation

862
Carbon dioxide fixation in prokaryotes enables the assimilation of inorganic carbon into organic molecules, supporting biosynthetic pathways, sustaining ecosystems, and contributing to the global carbon cycle. It also has industrial applications in carbon capture and bioproduct synthesis. Autotrophic organisms rely on this process to utilize CO₂ as a carbon source in diverse environments.The Calvin CycleThe Calvin cycle is the most widespread carbon fixation mechanism, primarily used by...
862

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Prediction of Prognosis and Immunotherapy of Osteosarcoma Based on Necroptosis-Related lncRNAs.

Frontiers in genetics·2022
Same author

Multiplex immunochromatographic platform based on crystal violet tag for simultaneous detection of streptomycin and chloramphenicol.

Food chemistry·2022
Same author

Biochemical recurrence related metabolic novel signature associates with immunity and ADT treatment responses in prostate cancer.

Cancer medicine·2022
Same author

Rate constants of chlorine atom reactions with organic molecules in aqueous solutions, an overview.

Environmental science and pollution research international·2022
Same author

Vanadium Disulfide Nanosheet Boosts Optical Signal Brightness as a Superior Enzyme Label to Improve the Sensitivity of Lateral Flow Immunoassay.

Analytical chemistry·2022
Same author

A TET1-PSPC1-Neat1 molecular axis modulates PRC2 functions in controlling stem cell bivalency.

Cell reports·2022

Related Experiment Video

Updated: Mar 24, 2026

Measurement of the Potential Rates of Dissimilatory Nitrate Reduction to Ammonium Based on 14NH4+/15NH4+ Analyses via Sequential Conversion to N2O
08:05

Measurement of the Potential Rates of Dissimilatory Nitrate Reduction to Ammonium Based on 14NH4+/15NH4+ Analyses via Sequential Conversion to N2O

Published on: October 7, 2020

6.7K

Biological denitrification using poly(butanediol succinate) as electron donor.

Zhiqiang Shen1,2, Yanan Yin1, Jianlong Wang3,4

  • 1Collaborative Innovation Center for Advanced Nuclear Energy Technology, INET, Tsinghua University, Beijing, 100084, People's Republic of China.

Applied Microbiology and Biotechnology
|March 11, 2016
PubMed
Summary

Poly(butanediol succinate) (PBS), a biodegradable polymer, effectively removed nitrate in a packed-bed bioreactor. This study highlights PBS as a promising carbon source and biofilm carrier for biological nitrate removal processes.

Keywords:
NitratePoly(butanediol succinate)denitrificationmicrobial communitysolid carbon source

More Related Videos

Estimating Sediment Denitrification Rates Using Cores and N2O Microsensors
07:59

Estimating Sediment Denitrification Rates Using Cores and N2O Microsensors

Published on: December 6, 2018

8.7K
Development of Sulfidogenic Sludge from Marine Sediments and Trichloroethylene Reduction in an Upflow Anaerobic Sludge Blanket Reactor
15:19

Development of Sulfidogenic Sludge from Marine Sediments and Trichloroethylene Reduction in an Upflow Anaerobic Sludge Blanket Reactor

Published on: October 15, 2015

10.2K

Related Experiment Videos

Last Updated: Mar 24, 2026

Measurement of the Potential Rates of Dissimilatory Nitrate Reduction to Ammonium Based on 14NH4+/15NH4+ Analyses via Sequential Conversion to N2O
08:05

Measurement of the Potential Rates of Dissimilatory Nitrate Reduction to Ammonium Based on 14NH4+/15NH4+ Analyses via Sequential Conversion to N2O

Published on: October 7, 2020

6.7K
Estimating Sediment Denitrification Rates Using Cores and N2O Microsensors
07:59

Estimating Sediment Denitrification Rates Using Cores and N2O Microsensors

Published on: December 6, 2018

8.7K
Development of Sulfidogenic Sludge from Marine Sediments and Trichloroethylene Reduction in an Upflow Anaerobic Sludge Blanket Reactor
15:19

Development of Sulfidogenic Sludge from Marine Sediments and Trichloroethylene Reduction in an Upflow Anaerobic Sludge Blanket Reactor

Published on: October 15, 2015

10.2K

Area of Science:

  • Environmental Science
  • Microbiology
  • Polymer Science

Background:

  • Nitrate contamination in water bodies poses significant environmental and health risks.
  • Biological nitrate removal processes often require an effective carbon source and biofilm carrier.
  • Biodegradable polymers offer potential sustainable solutions for wastewater treatment.

Purpose of the Study:

  • To evaluate Poly(butanediol succinate) (PBS) as a solid carbon source and biofilm carrier for biological nitrate removal.
  • To assess the denitrification performance of a packed-bed bioreactor using PBS.
  • To investigate the microbial diversity of the biofilm formed on PBS.

Main Methods:

  • A packed-bed bioreactor was filled with Poly(butanediol succinate) (PBS).
  • The bioreactor was operated for biological nitrate removal, and performance was monitored.
  • Biofilm samples attached to PBS were analyzed for microbial community structure using pyrosequencing.

Main Results:

  • The volumetric denitrification rate achieved was 0.60 kg m⁻³ day⁻¹ at a loading rate of 0.63 kg m⁻³ day⁻¹.
  • Effluent nitrite nitrogen (NO2-N) concentration remained below 0.20 mg L⁻¹.
  • The effluent pH showed a slight decrease from 6.98-7.87 to 6.46-7.18.
  • Proteobacteria (89.87%) was the dominant phylum in the biofilm, with β-Proteobacteria as the most abundant class.
  • Key genera identified included Dechloromonas, Alicycliphilus, Azospira, and Sinobacteraceae-uncultured.

Conclusions:

  • Poly(butanediol succinate) (PBS) is a suitable and promising alternative solid carbon source for biological nitrate removal.
  • PBS effectively supports biofilm formation and denitrification in a packed-bed bioreactor.
  • The microbial community structure is dominated by Proteobacteria, indicating efficient biological processes.