Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Tagging and Fusion Proteins

Tagging and Fusion Proteins

Proteins are involved in several cellular processes and biochemical reactions. Analyzing a specific protein of interest requires it to be isolated from the other proteins in the cell. This is achieved by overexpressing the specific gene in a suitable host to produce large quantities of the target protein. A tag or label is recombined with the gene to produce a fusion protein containing the target protein and the tag. The tags on these fusion proteins can then be used for easy detection and...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

The influence of substrate and ethanol treatment on properties and quantitative analysis of retention of recombinant spider silk coatings.

International journal of biological macromolecules·2026

Same author

Temperature and time induced assembly phase changes of engineered spidroin protein solutions.

International journal of biological macromolecules·2025

Same author

Upcycling Agricultural Waste for Functional Interfaces: Yellow Onion Skin-Derived Dyes for Cellulosic Materials.

ACS omega·2025

Same author

Multiscale interface engineering enables strong and water resistant wood bonding.

Nature communications·2025

Same author

Hydrophobin-Coated Echogenic Microbubbles for Molecular Targeting of Tumor Cells.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2025

Same author

Nanobolometer with ultralow noise equivalent power.

Communications physics·2025

Search research articles

Related Experiment Video

Updated: Mar 24, 2026

Development and Functionalization of Electrolyte-Gated Graphene Field-Effect Transistor for Biomarker Detection

Development and Functionalization of Electrolyte-Gated Graphene Field-Effect Transistor for Biomarker Detection

Published on: February 1, 2022

Graphene Biosensor Programming with Genetically Engineered Fusion Protein Monolayers.

Miika Soikkeli¹, Katri Kurppa¹, Markku Kainlauri¹

¹VTT Technical Research Centre of Finland Ltd. , P.O. Box 1000, FI-02044 VTT, Espoo, Finland.

ACS Applied Materials & Interfaces

|March 11, 2016

Summary

This study presents a novel label-free biosensor using graphene field-effect transistors and specific receptor modules for highly sensitive and rapid detection of analytes. The innovative design allows for easy functionalization and in situ replacement of receptor monolayers, enhancing device versatility and shelf life.

Keywords:

Debye length biosensor fusion protein graphene hydrophobin self-assembly

More Related Videos

Exploring Biomolecular Interaction Between the Molecular Chaperone Hsp90 and Its Client Protein Kinase Cdc37 using Field-Effect Biosensing Technology

Exploring Biomolecular Interaction Between the Molecular Chaperone Hsp90 and Its Client Protein Kinase Cdc37 using Field-Effect Biosensing Technology

Published on: March 31, 2022

Bridging the Bio-Electronic Interface with Biofabrication

Bridging the Bio-Electronic Interface with Biofabrication

Published on: June 6, 2012

Related Experiment Videos

Last Updated: Mar 24, 2026

Development and Functionalization of Electrolyte-Gated Graphene Field-Effect Transistor for Biomarker Detection

Development and Functionalization of Electrolyte-Gated Graphene Field-Effect Transistor for Biomarker Detection

Published on: February 1, 2022

Exploring Biomolecular Interaction Between the Molecular Chaperone Hsp90 and Its Client Protein Kinase Cdc37 using Field-Effect Biosensing Technology

Exploring Biomolecular Interaction Between the Molecular Chaperone Hsp90 and Its Client Protein Kinase Cdc37 using Field-Effect Biosensing Technology

Published on: March 31, 2022

Bridging the Bio-Electronic Interface with Biofabrication

Bridging the Bio-Electronic Interface with Biofabrication

Published on: June 6, 2012

Area of Science:

Biotechnology
Nanotechnology
Materials Science

Background:

Label-free biosensors are crucial for real-time molecular detection.
Graphene field-effect transistors (GFETs) offer high sensitivity for charge-based sensing.
Developing robust and versatile functionalization methods for GFET biosensors remains a challenge.

Purpose of the Study:

To demonstrate a novel label-free biosensor concept utilizing specific receptor modules and GFETs.
To develop a simple and efficient method for sensor functionalization and regeneration.
To assess the performance of the biosensor for detecting various biomolecules.

Main Methods:

Engineered fusion proteins (receptor modules) with hydrophobin anchors for immobilization.
Single-step, directed self-assembly of receptor modules onto hydrophobic graphene surfaces.
Charge sensing using a graphene field-effect transistor (GFET).
In situ removal and replacement of receptor monolayers for versatile selectivity.

Main Results:

Achieved femtomolar sensitivity for detecting small charged peptides and large immunoglobulin molecules.
Demonstrated a fast response time, on the order of one second.
Confirmed the stability of receptor monolayers upon drying, indicating a reasonable shelf life for the functionalized devices.
Showcased the ability to replace receptor monolayers in situ, enabling adaptable sensor selectivity.

Conclusions:

The developed GFET-based biosensor offers a label-free, highly sensitive, and rapid detection platform.
The receptor module strategy provides a versatile and stable approach for biosensor functionalization and regeneration.
This technology holds promise for various applications requiring sensitive and adaptable biomolecule detection.