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MicroRNA-183 Functions As an Oncogene by Regulating PDCD4 in Gastric Cancer

Chenglong Li, Li Deng, Qiaoming Zhi

  • 1Department of General Surgery and Translational Medicine Center, Nanjing Medical University Affiliated Wuxi Second Hospital, Wuxi, 214002, China. jiazengxia@yahoo.com.

Anti-Cancer Agents in Medicinal Chemistry
|March 11, 2016
PubMed

Insights

MicroRNA-183 (miR-183) is over-expressed in gastric cancer (GC), promoting tumor growth and metastasis. Inhibiting miR-183 suppressed GC cell progression by targeting the PDCD4 gene, suggesting miR-183 acts as an oncogene in GC.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • MicroRNA-183 (miR-183) plays a role in various human cancers, but its function in gastric cancer (GC) is not well understood.
  • Gastric cancer remains a significant global health challenge with a need for better understanding of its molecular drivers.

Purpose of the Study:

  • To investigate the role and mechanism of miR-183 in gastric cancer.
  • To determine the association between miR-183 expression and GC clinicopathological features.

Main Methods:

  • Quantitative real-time PCR to measure miR-183 expression in GC tissues and cells.
  • In vitro assays including cell proliferation, colony formation, migration, invasion, adhesion, and apoptosis assays.
  • Western blot and luciferase reporter assays to validate PDCD4 as a direct target of miR-183.

Main Results:

  • miR-183 was significantly over-expressed in GC tissues and associated with advanced clinicopathological characteristics.
  • Inhibition of endogenous miR-183 in GC cells reduced proliferation, colony formation, migration, invasion, and adhesion, while enhancing apoptosis.
  • PDCD4 was identified as a direct downstream target of miR-183 in GC cells.

Conclusions:

  • miR-183 functions as an oncogene in gastric cancer by promoting cell proliferation, survival, and metastasis.
  • The oncogenic role of miR-183 in GC is mediated, at least in part, through the direct targeting of the tumor suppressor gene PDCD4.

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