Pontibacter mucosus sp. nov., isolated from hexachlorocyclohexane-contaminated pond sediment

A halotolerant, Gram-stain-negative, rod-shaped and light-pink-pigmented bacterial strain, PB3T, was isolated from a pond sediment near a hexachlorocyclohexane-producing factory, located at Chinhat, Lucknow, India. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain PB3T formed a distinct phyletic clade along with the members of the genus Pontibacter. The 16S rRNA gene sequence similarity with other members of the genus Pontibacter ranged from 94.5 to 98.9 %. The cells were motile, aerobic, and catalase- and oxidase-positive. The major fatty acids were iso-C15:0, iso-C15:0 3-OH, iso-C17:0 3-OH, C16:1ω5c, summed feature 3 (C16:1ω6c/C16:1ω7c) and summed feature 4 (iso-C17:1I/ anteiso-C17:1 B). The polar lipid profile of strain PB3T showed the presence of phosphatidylethanolamine, an unidentified aminophospholipid, unknown aminolipids and other unknown polar lipids. DNA-DNA hybridization based homology of strain PB3T with respect to its most closely related species, Pontibacter chinhatensis LP51T, was 44.7 %. The DNA G+C content was 53.5 mol%. On the basis of these data, it is proposed that the isolate belongs to the genus Pontibacter and represents a novel species, for which the name Pontibacter mucosus is proposed. The type strain is PB3T (=DSM 100162T=KCTC 42942T).