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Updated: Mar 24, 2026
08:11
Assessing the Innate Sensing of HIV-1 Infected CD4+ T Cells by Plasmacytoid Dendritic Cells Using an Ex vivo Co-culture System.
Published on: September 1, 2015
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Actin' on HIV: How Dendritic Cells Spread Infection
Daniel A Donahue1, Olivier Schwartz2
1Institut Pasteur, Department of Virology, Virus & Immunity Unit, Paris 75015, France; CNRS URA 3015, Paris 75015, France.
Cell Host & Microbe
|March 11, 2016
Summary
Dendritic cells (DCs) capture HIV and transfer it to CD4 T cells. Actin regulation in DCs is crucial for HIV uptake and transfer via membrane protrusions.
Area of Science:
- Immunology
- Virology
- Cell Biology
Background:
- Dendritic cells (DCs) are key in HIV transmission to CD4 T cells.
- Understanding DC mechanisms in HIV capture and transfer is vital for therapeutic strategies.
Purpose of the Study:
- To identify cellular factors in DCs that regulate HIV uptake and transfer.
- To investigate the role of actin dynamics in DC-mediated HIV transmission.
Main Methods:
- Conducted an shRNA screen in dendritic cells.
- Analyzed the impact of actin nucleation and stabilization on HIV particle capture.
- Examined the role of membrane protrusions in viral transfer.
Main Results:
- Actin nucleation and stabilization were found to regulate HIV uptake by DCs.
- These actin dynamics maintain HIV particles on membrane protrusions.
- This mechanism facilitates efficient HIV transfer to CD4 T cells.
Conclusions:
- Actin cytoskeleton remodeling is critical for dendritic cell function in HIV transmission.
- Targeting actin dynamics in DCs could offer novel strategies to control HIV spread.
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