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Published on: July 20, 2022
EFFECTS CRYOPRESERVATION OF IONOTROPIC GLUTAMATERGIC RECEPTOR MECHANISMS IN VITRO
1I. P. Pavlov Institute of Physiology, Russian Academy of Science, Saint-Petersburg, Russia. mok@inbox.ru.
Background:
We assayed hypothesis of cryopreservation of small volumes of nervous tissue (olfactory cortex slices).
Objective:
Effect of freezing (-10(0)C) and rewarming to +37(0)C studied on glutamatergic mechanisms.
Materials And Methods:
Viability slices determined from the change of the amplitudes of glutamatergic synaptic transmission: action potential of lateral olfactory tract (AP LOT), α-amino-3-hydroxy-5-methylisoxazole-4-propionic acid (AMPAR) and N-methyl-D-aspartate (NMDAR) receptor-dependent mechanisms. Effects of two rates freezing/rewarming: slow (0.1(0)C/min) and rapid (9.0(0)C/min) were studied.
Results:
Activity AP LOT and AMPARs restored at slow rate freezing/rewarming, and at rapid rate, these indicators were increased. Activity NMDARs decreased at slow rate both at slow and at rapid rates after freezing/rewarming were blocked. At rewarming water swelling content ofin slices was maximal at rapid rate of freezing/rewarming.
Conclusion:
The protocol of cryopreservation with slow rate (0.1(0)C/min) freezing/rewarming and a depth of freezing -10(0)C is optimal for the cryopreservation of small volumes of nervous tissue.
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