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Sequence comparison of six human microRNAs genes between tuberculosis patients and healthy individuals

A Amila1, A Acosta2, M E Sarmiento1

  • 1School of Health Sciences, Universiti Sains Malaysia, Kubang Kerian, Kelantan, Malaysia.

International Journal of Mycobacteriology
|March 12, 2016
PubMed
Abstract

Insights

Genetic variations in specific human microRNAs (miRNAs) were investigated for their association with pulmonary tuberculosis (TB). No significant sequence differences were found in the selected miRNAs between TB patients and healthy individuals.

Area of Science:

  • Genetics
  • Molecular Biology
  • Immunology

Background:

  • MicroRNAs (miRNAs) are crucial regulators in disease development.
  • Human miRNAs can potentially inhibit Mycobacterium tuberculosis (Mtb) survival by targeting pathogen genes.
  • Mutations in miRNAs may impair Mtb gene inhibition, increasing susceptibility to tuberculosis (TB).

Purpose of the Study:

  • To investigate the genetic association between pulmonary TB and six specific human miRNA genes.
  • To determine if sequence variations in selected miRNAs correlate with the inhibition of critical Mtb genes in TB patients.

Main Methods:

  • Examined genetic association of pulmonary TB with hsa-miR-370, hsa-miR-520d, hsa-miR-154, hsa-miR-497, hsa-miR-758, and hsa-miR-593.
  • Focused on sequence variations in miRNAs predicted to interact with Mtb genes.

Main Results:

  • No significant differences in miRNA sequences were observed between TB patients and healthy individuals.
  • The study did not find a genetic association between the selected miRNA gene sequences and pulmonary TB.

Conclusions:

  • The lack of observed genetic association may be due to sample size limitations.
  • Future studies should address sample size and explore a broader selection of miRNA genes for a comprehensive understanding.
Keywords:
Mycobacterium tuberculosisTuberculosismicroRNAs

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