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Related Experiment Video

Updated: Mar 24, 2026

In Vivo Intracerebral Stereotaxic Injections for Optogenetic Stimulation of Long-Range Inputs in Mouse Brain Slices

In Vivo Intracerebral Stereotaxic Injections for Optogenetic Stimulation of Long-Range Inputs in Mouse Brain Slices

Published on: September 20, 2019

Optogenetic Approaches for Mesoscopic Brain Mapping.

Michael Kyweriga¹, Majid H Mohajerani²

¹Department of Neuroscience, Canadian Centre for Behavioural Neuroscience, University of Lethbridge at Lethbridge, 4401 University Dr W., Lethbridge, AB, Canada, T1K 3M4.

Methods in Molecular Biology (Clifton, N.J.)

|March 12, 2016

Summary

This summary is machine-generated.

This guide details optogenetics, a technique using light-sensitive opsins to control specific neurons. It provides practical instructions for implementing these advanced brain research methods in any laboratory setting.

Keywords:

Brain Channelrhodopsin (ChR2)Circuit Mouse Neuron Optogenetics Transgenic Virus Voltage-sensitive dyes (VSD)

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Last Updated: Mar 24, 2026

In Vivo Intracerebral Stereotaxic Injections for Optogenetic Stimulation of Long-Range Inputs in Mouse Brain Slices

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Published on: September 20, 2019

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A Large Lateral Craniotomy Procedure for Mesoscale Wide-field Optical Imaging of Brain Activity

A Large Lateral Craniotomy Procedure for Mesoscale Wide-field Optical Imaging of Brain Activity

Published on: May 7, 2017

Area of Science:

Neuroscience
Molecular Biology
Genetics

Background:

Genetically unique neuronal proteins enable advanced brain circuitry studies.
Opsins can be inserted into specific cell types using viral vectors or transgenic mice.
Optogenetics allows precise control of neuronal activity with millisecond timing.

Purpose of the Study:

To provide a practical guide for implementing optogenetic techniques in research labs.
To detail the materials, considerations, and surgical procedures for optogenetic experiments.
To introduce all-optical mapping for simultaneous neural recording and manipulation.

Main Methods:

Viral injections and transgenic mouse breeding for opsin delivery.
Acute surgical procedures in mice for targeted optogenetic manipulation.
All-optical mapping combining optogenetic stimulation and voltage-sensitive dye imaging.

Main Results:

Successful implementation of optogenetics for precise neuronal control.
Demonstration of all-optical techniques for population activity mapping in vivo.
Establishment of a comprehensive protocol for laboratory use.

Conclusions:

Optogenetics offers a powerful tool for dissecting neural circuits.
This guide democratizes access to advanced optogenetic methods.
The presented techniques facilitate in vivo recording and manipulation of neural populations.