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Updated: Mar 24, 2026

In vivo Optogenetic Stimulation of the Rodent Central Nervous System

In vivo Optogenetic Stimulation of the Rodent Central Nervous System

Published on: January 15, 2015

A Multichannel Recording System with Optical Stimulation for Closed-Loop Optogenetic Experiments.

Carmen Bartic¹, Francesco P Battaglia², Ling Wang³

¹Department of Physics and Astronomy, KU Leuven, Celestijnenlaan 200D, Heverlee, 3001, Belgium. carmen.bartic@fys.kuleuven.be.

Methods in Molecular Biology (Clifton, N.J.)

|March 12, 2016

Summary

This summary is machine-generated.

This study introduces optogenetics, a method using light to control specific brain cells. It details tools and protocols for real-time, closed-loop experiments in vivo to understand brain circuits.

Keywords:

Closed-loop Multichannel recordings Optic fibers Optical stimulation Spike-sorting Tetrode

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Last Updated: Mar 24, 2026

In vivo Optogenetic Stimulation of the Rodent Central Nervous System

In vivo Optogenetic Stimulation of the Rodent Central Nervous System

Published on: January 15, 2015

Optrode Array for Simultaneous Optogenetic Modulation and Electrical Neural Recording

Optrode Array for Simultaneous Optogenetic Modulation and Electrical Neural Recording

Published on: September 1, 2022

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Published on: November 21, 2024

Area of Science:

Neuroscience
Optogenetics
Systems Neuroscience

Background:

Understanding neuronal circuits is key for cognition, behavior, and neuromodulation.
Optogenetics enables selective cell targeting via light-sensitive opsins and genetic methods.

Purpose of the Study:

To describe essential tools and protocols for closed-loop optogenetic experiments in vivo.
To facilitate real-time modulation and monitoring of neuronal activity.

Main Methods:

Utilizing optogenetics for optical activation of opsin-expressing cells.
Employing microelectrode arrays for monitoring electrical activity.
Implementing closed-loop systems for real-time adjustment of optical stimulation parameters.

Main Results:

Successful demonstration of basic tools for in vivo optogenetics.
Establishment of protocols for closed-loop optogenetic experiments.
Enabling real-time feedback control of neuronal activity.

Conclusions:

Closed-loop optogenetics provides a powerful approach for studying brain function.
The described methods and tools support in vivo investigation of neuronal circuits.
This technique holds potential for therapeutic neuromodulation strategies.