Related Experiment Video

Updated: Mar 24, 2026

Light-mediated Reversible Modulation of the Mitogen-activated Protein Kinase Pathway during Cell Differentiation and Xenopus Embryonic Development
09:32

Light-mediated Reversible Modulation of the Mitogen-activated Protein Kinase Pathway during Cell Differentiation and Xenopus Embryonic Development

Published on: June 15, 2017

9.3K

Optogenetic Control of Fibroblast Growth Factor Receptor Signaling

Nury Kim1, Jin Man Kim2, Won Do Heo3,4

  • 1Center for Cognition and Sociality, Institute for Basic Science, Seoul, 136-791, Republic of Korea.

Methods in Molecular Biology (Clifton, N.J.)
|March 12, 2016
PubMed

Insights

This study introduces OptoFGFR1, a novel optogenetic tool to control fibroblast growth factor receptor 1 (FGFR1) signaling with light. This method allows precise manipulation of FGFR1 to investigate its role in cell functions like migration.

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Optogenetics

Background:

  • Fibroblast growth factor receptor 1 (FGFR1) is crucial for cellular functions including proliferation, migration, and differentiation.
  • Modulating FGFR1 signaling is essential for understanding its role in biological processes.
  • Existing methods lack precise spatiotemporal control over FGFR1 signaling.

Purpose of the Study:

  • To develop and validate OptoFGFR1, an optogenetic tool for light-guided manipulation of FGFR1 signaling.
  • To investigate the downstream effects of FGFR1 activation on cellular processes.
  • To enable precise control of FGFR1 signaling in single cells or cell populations.

Main Methods:

  • Development of OptoFGFR1 by fusing the cryptochrome2 PHR domain with the FGFR1 cytoplasmic region.
  • Utilizing optogenetic techniques for light-induced activation of FGFR1.
  • Employing confocal microscopy and LED arrays for spatiotemporal control of FGFR1 signaling.

Main Results:

  • OptoFGFR1 enables precise, light-dependent activation of the FGFR1 signaling pathway.
  • Demonstrated spatiotemporal control of FGFR1 signaling in single cells and cell groups.
  • Successfully applied OptoFGFR1 to study light-guided cell migration.

Conclusions:

  • OptoFGFR1 provides a powerful new tool for studying FGFR1 signaling with high spatiotemporal resolution.
  • This optogenetic approach facilitates the investigation of FGFR1's role in diverse cellular functions.
  • OptoFGFR1 opens avenues for exploring FGFR1-mediated processes in complex biological systems.
Keywords:
FGFRLive cell imagingMigrationOptogeneticsSignaling pathwaySpatiotemporal regulationoptoFGFR1

More Related Videos

Suppression of Pro-fibrotic Signaling Potentiates Factor-mediated Reprogramming of Mouse Embryonic Fibroblasts into Induced Cardiomyocytes09:16

Suppression of Pro-fibrotic Signaling Potentiates Factor-mediated Reprogramming of Mouse Embryonic Fibroblasts into Induced Cardiomyocytes

Published on: June 3, 2018

7.8K
Spatiotemporal Control of Protein Activity through Optogenetic Allosteric Regulation08:00

Spatiotemporal Control of Protein Activity through Optogenetic Allosteric Regulation

Published on: October 4, 2024

1.2K

Related Concept Videos

TGF - β Signaling Pathway01:16

TGF - β Signaling Pathway

The TGF-β signaling pathway regulates cell growth, differentiation, adhesion, motility, and development. TGF-β ligands that induce TGF-β signaling are synthesized in their latent form. Several proteases or cell surface receptors such as integrins act upon the latent form, releasing the active ligand. There are three types of mammalian TGF-βs: (TGF-β1, TGF-β2, and TGF-β3) that bind as homodimers or heterodimers to TGF-β receptors. The TGF-β receptors...
10.9K
Introduction to Fibroblasts01:09

Introduction to Fibroblasts

Rudolph Virchow discovered spindle-shaped cells called fibroblasts in 1858. Inactive fibroblasts, called fibrocytes, become activated by various stimuli, such as growth factors and inflammatory cytokines. Activated fibroblasts play a crucial role in wound healing, inflammation, formation of new blood vessels, and cancer progression. Uncontrolled activation of fibroblasts results in fibrosis, the excess deposition of fibrous tissue, which can lead to scarring and affect normal organs. This...
4.3K
Regulation of Angiogenesis and Blood Supply01:24

Regulation of Angiogenesis and Blood Supply

Rapidly dividing tumors, embryos, and wounded tissues require more oxygen than usual, lowering the oxygen concentration in the blood. At low oxygen or hypoxic conditions, an oxygen-sensitive transcription factor called the hypoxia-inducible factor 1 or HIF1 is activated. HIF1 is a dimeric protein of alpha (ɑ) and beta (β) subunits.  Under optimal oxygen conditions, HIF1β is present in the nucleus while HIF1ɑ remains in the cytosol. HIF1ɑ is hydroxylated by prolyl...
3.9K