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Updated: Mar 24, 2026

Global Identification of Co-Translational Interaction Networks by Selective Ribosome Profiling
Published on: October 7, 2021
Ribo-Proteomics Approach to Profile RNA-Protein and Protein-Protein Interaction Networks
Hsin-Sung Yeh1, Jae-Woong Chang1, Jeongsik Yong2
1Department of Biochemistry, Molecular Biology and Biophysics, University of Minnesota Twin Cities, 6-155 Jackson Hall, 321 Church Street SE, Minneapolis, MN, 55455, USA.
Abstract:
Characterizing protein-protein and protein-RNA interaction networks is a fundamental step to understanding the function of an RNA-binding protein. In many cases, these interactions are transient and highly dynamic. Therefore, capturing stable as well as transient interactions in living cells for the identification of protein-binding partners and the mapping of RNA-binding sequences is key to a successful establishment of the molecular interaction network. In this chapter, we will describe a method for capturing the molecular interactions in living cells using formaldehyde as a crosslinker and enriching a specific RNA-protein complex from cell extracts followed by mass spectrometry and Next-Gen sequencing analyses.
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