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Establishing Organoids from Human Tooth as a Powerful Tool Toward Mechanistic Research and Regenerative Therapy

Establishing Organoids from Human Tooth as a Powerful Tool Toward Mechanistic Research and Regenerative Therapy

Published on: April 13, 2022

Three-dimensional Micro-culture System for Tooth Tissue Engineering.

S Kuchler-Bopp¹, T Bécavin², T Kökten³

¹INSERM, Unité Mixte de Recherche UMR 1109, Team "Osteoarticular and Dental Regenerative NanoMedicine," Fédération de Médecine Translationnelle de Strasbourg, UDS, Strasbourg, France Faculté de Chirurgie Dentaire, UDS, Strasbourg, France kuchler@unistra.fr.

Journal of Dental Research

|March 12, 2016

Summary

This summary is machine-generated.

Researchers developed a novel hanging drop culture method for regenerating teeth. This technique uses fewer cells and successfully produced mineralized teeth with innervation after in vivo implantation.

Keywords:

E-cadherin epithelial-mesenchymal interaction hanging drop microtissue odontogenesis spheroids

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Area of Science:

Developmental Biology
Tissue Engineering
Regenerative Medicine

Background:

Cellular arrangement is crucial for organogenesis, particularly in tooth development.
Current research focuses on regenerating teeth by reassociating embryonic dental cells and in vivo implantation.

Purpose of the Study:

To evaluate the hanging drop method for studying mixed epithelial-mesenchymal cell reorganization in liquid culture for tooth histogenesis and organogenesis.
To optimize protocols for in vitro tooth development and subsequent in vivo implantation.

Main Methods:

Utilized the hanging drop method for culturing mixed epithelial-mesenchymal dental cells in a liquid environment.
Compared three different culture protocols, including sequential combinations of hanging drop and semisolid cultures.
Assessed tooth development, mineralization, vascularization, cellular heterogeneity, and innervation following in vivo implantation.

Main Results:

The sequential hanging drop and semisolid culture protocol yielded the best results for tooth regeneration.
Implanted tissues developed into well-formed teeth with mineralized dentin and enamel, and initial root formation.
Co-implantation with trigeminal ganglion resulted in innervation of the dental mesenchyme and odontoblast layer.

Conclusions:

The hanging drop method offers a controlled environment favoring cell migration for tooth organogenesis.
This technique requires a minimal number of cells, making it an efficient model for tooth regeneration.
The developed experimental model is suitable for studying tooth development, physiology, metabolism, toxicology, and testing alternative cell sources.