Intervolume analysis to achieve four-dimensional optical microangiography for observation of dynamic blood flow
Wei Wei1, Jingjiang Xu1, Utku Baran2
1University of Washington, Department of Bioengineering, 3720 15th Avenue NE, Seattle, Washington 98195, United States.
Abstract:
We demonstrate in vivo volumetric optical microangiography at ∼ 200 volumes/s by the use of 1.6 MHz Fourier domain mode-locking swept source optical coherence tomography and an effective 36 kHz microelectromechanical system (MEMS) scanner. We propose an intervolume analysis strategy to contrast the dynamic blood flow signal from the static tissue background. The proposed system is demonstrated by imaging cerebral blood flow in mice in vivo. For the first time, imaging speed, sensitivity, and temporal resolution become possible for a direct four-dimensional observation of microcirculations within live body parts.
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