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Intravital Microscopy of Tumor-associated Vasculature Using Advanced Dorsal Skinfold Window Chambers on Transgenic Fluorescent Mice

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In vivo microscopy.

János Peti-Peterdi¹

¹Departments of Physiology and Biophysics, and Medicine, Zilkha Neurogenetic Institute, ZNI355, University of Southern California, 1501 San Pablo Street, Los Angeles, CA 90033, USA.

Nephrologie & Therapeutique

|March 13, 2016

Summary

Multiphoton microscopy advances intravital kidney imaging, offering insights into kidney function and disease mechanisms. This technique aids in understanding glomerular pathologies and cell behavior in living kidneys.

Keywords:

Albumin leakage Confetti construct GCaMP Glomerular filtration barrier Multiphoton microscopy Podocyte Purinergic signaling

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Area of Science:

Nephrology
Microscopy
Physiology

Background:

Multiphoton excitation fluorescence microscopy has been instrumental in studying kidney physiology and disease over the last 15 years.
Intravital imaging provides a unique window into the dynamic processes within the living kidney.

Purpose of the Study:

To review the applications of multiphoton microscopy for intravital kidney imaging.
To highlight its role in understanding kidney function, disease mechanisms, and glomerular pathologies.

Main Methods:

Utilizing multiphoton excitation fluorescence microscopy for real-time imaging of the kidney.
Combining intravital multiphoton imaging with mouse genetics for detailed cellular and molecular analysis.
Employing serial imaging of the same glomerulus over time in live animals.

Main Results:

Demonstrated the utility of multiphoton imaging in studying the glomerulus and glomerular filtration barrier.
Showcased how combining imaging with mouse models elucidates glomerular cell types, functions, and disease mechanisms.
Highlighted serial imaging's potential for longitudinal studies of kidney health and disease.

Conclusions:

Multiphoton microscopy is a powerful tool for intravital kidney research, advancing nephrology.
The technique facilitates a deeper understanding of glomerular structure, function, and disease.
Future applications promise further breakthroughs in kidney research and treatment strategies.