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Related Experiment Video

Updated: Mar 24, 2026

In vivo Evaluation of Mucociliary Clearance in Mice

In vivo Evaluation of Mucociliary Clearance in Mice

Published on: December 18, 2020

Reduced mucociliary clearance in old mice is associated with a decrease in Muc5b mucin.

Barbara R Grubb¹, Alessandra Livraghi-Butrico¹, Troy D Rogers¹

¹Marsico Lung Institute/UNC CF Research Center, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina; and.

American Journal of Physiology. Lung Cellular and Molecular Physiology

|March 13, 2016

Summary

This summary is machine-generated.

Aging impairs mucociliary clearance (MCC) in mice due to reduced chloride secretion and lower levels of the essential mucin Muc5b, increasing respiratory infection risk in the elderly.

Keywords:

aging mucociliary clearance

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Area of Science:

Respiratory Physiology
Aging Research
Immunology

Background:

Respiratory infections pose significant risks to the elderly.
Mucociliary clearance (MCC) is vital for airway defense.
Previous studies suggest age-related decline in MCC, but underlying mechanisms remain unclear.

Purpose of the Study:

To investigate the mechanisms behind age-associated decline in MCC.
To compare MCC function in young and old C57BL/6 mice.

Main Methods:

Assessed MCC function in upper and lower airways of young and old mice.
Measured bioelectric properties of isolated tracheal and bronchial tissues.
Quantified levels of secreted mucin Muc5b in bronchioalveolar lavage and tissue samples.

Main Results:

Old mice exhibited significantly reduced MCC function compared to young mice.
A decrease in chloride secretion was observed in older mice.
Reduced levels of the major secreted mucin, Muc5b, were found in aged mice, correlating with impaired MCC.

Conclusions:

Aged mice show diminished MCC function.
Reduced secretion of chloride and Muc5b are likely contributors to age-related MCC impairment.
These findings highlight potential therapeutic targets for respiratory health in the elderly.