Related Experiment Video
Updated: Mar 24, 2026
A High-throughput Compatible Assay to Evaluate Drug Efficacy against Macrophage Passaged Mycobacterium tuberculosis
Published on: March 24, 2017
Rapid colorimetric pyrazinamide susceptibility testing of Mycobacterium tuberculosis
1Key Laboratory of Special Pathogens and Biosafety, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, China; University of Chinese Academy of Sciences, Beijing, China.
Setting:
Colorimetric methods for detecting drug resistance in Mycobacterium tuberculosis are very attractive, as they are cheap, easy to use and require no costly apparatus. Although the current colorimetric methods have been used for other drugs, such as rifampicin and isoniazid, few of the colorimetric methods have been reported to have been used in performing drug susceptibility testing (DST) against pyrazinamide (PZA).
Objective:
To develop a rapid and reliable colorimetric method for PZA DST using a monotetrazolium redox dye, 5-cyano-2,3-ditolyl tetrazolium chloride (CTC), as indicator of viability.
Design:
A total of 50 clinical isolates and three standard strains were tested using this colorimetric method and the BACTEC™ MGIT™ 960 system.
Results:
Initial test results showed that the PZA DST results were available in 4-6 days; the overall sensitivity and specificity of the CTC colorimetric method were respectively 97.1% and 81.3% in comparison with the MGIT 960 results.
Conclusion:
These results suggest that the CTC colorimetric method is most rapid among the current PZA DST methods based on culture, and could be used for determining susceptibility to PZA of M. tuberculosis isolates.
More Related Videos
09:57System for Efficacy and Cytotoxicity Screening of Inhibitors Targeting Intracellular Mycobacterium tuberculosis
Published on: April 5, 2017
12:39Antimicrobial Susceptibility Testing of Mycobacterium Tuberculosis Complex for First and Second Line Drugs by Broth Dilution in a Microtiter Plate Format
Published on: June 24, 2011
