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Updated: Mar 24, 2026

The MultiBac Protein Complex Production Platform at the EMBL
Published on: July 11, 2013
How to Build a Complex, Functional Propeller Protein, From Parts
1Department of Chemistry and Biochemistry, Department of Chemical and Biomolecular Engineering, University of Notre Dame, Notre Dame, IN 46556, USA.
Abstract:
By combining ancestral sequence reconstruction and in vitro evolution, Smock et al. identified single motifs that assemble into a functional five-bladed β-propeller, and a likely route for conversion into the more complex, extant single chain fusion. Interestingly, although sequence diversification destabilized five-motif fusions, it also destabilized aggregation-prone intermediates, increasing the level of functional protein in vivo.
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