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Pdgfra and Pdgfrb genetically interact during craniofacial development.

Neil McCarthy1, Jocelyn S Liu2, Alicia M Richarte3

  • 1Department of Molecular Biosciences, Institute for Cellular and Molecular Biology, Institute for Neuroscience, Waggoner Center for Alcohol and Addiction Research, University of Texas, Austin, Texas.

Developmental Dynamics : an Official Publication of the American Association of Anatomists
|March 15, 2016
PubMed
Summary

Platelet-derived growth factor receptors (Pdgfra and Pdgfrb) interact during palate development. This interaction is crucial for proper neural crest cell condensation, impacting palatogenesis in both zebrafish and mouse models.

Keywords:
cranial neural crestpalatogenesispdgfrapdgfrb

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Area of Science:

  • Developmental Biology
  • Craniofacial Development
  • Molecular Biology

Background:

  • Cleft palate is a common congenital birth defect.
  • Platelet-derived growth factors (Pdgf) are essential for palatogenesis.
  • Pdgfra is known to be required for neural crest migration and palate formation.

Purpose of the Study:

  • To investigate the role of pdgfrb in neural crest development.
  • To determine if pdgfra and pdgfrb interact during palatogenesis.

Main Methods:

  • Zebrafish and mouse models were used.
  • Analysis of pdgfra and pdgfrb single and double mutants.
  • Time-lapse confocal microscopy and pharmacological analyses were employed.

Main Results:

  • Pdgfrb is dispensable for craniofacial development in zebrafish.
  • Palatal defects in pdgfra;pdgfrb double mutants were more severe than in pdgfra single mutants.
  • A failure in neural crest condensation was observed in double mutants, indicating an interaction between Pdgfra and Pdgfrb.

Conclusions:

  • Pdgfra and Pdgfrb interact during palatogenesis in both zebrafish and mouse.
  • This interaction is critical for the proper condensation of maxillary neural crest cells.
  • A novel interaction between Pdgfra and Pdgfrb in palate formation has been identified.