Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Bacterial Translocation and Protein Secretion01:26

Bacterial Translocation and Protein Secretion

978
Bacterial protein secretion involves translocation systems to ensure proteins reach their designated locations, including the plasma membrane, periplasm, outer membrane, or the external environment. These translocation systems are vital for bacterial physiology, supporting processes like membrane assembly, enzymatic activity in the periplasm, and interactions with the external environment. The division of labor between Sec and Tat pathways ensures efficiency in handling proteins with diverse...
978
Protein Translocation Machinery on the ER Membrane01:28

Protein Translocation Machinery on the ER Membrane

7.3K
The translocon complex situated on the ER membrane is the main gateway for the protein secretory pathway. It facilitates the transport of nascent peptides into the ER lumen and their insertion into the ER membrane.
Sec61 protein conducting channel
In eukaryotes, the translocon complex comprises a core heterotrimeric translocator channel called the Sec61 complex. This channel includes three transmembrane proteins, Sec61α, Sec61β, and Sec61γ, and is the largest subunit of the...
7.3K
Cotranslational Protein Translocation01:20

Cotranslational Protein Translocation

10.9K
Translocation of proteins across membranes is an ancient process that occurs even in bacteria and archaebacteria. In fact, the components of the translocation machinery are still conserved between prokaryotes and eukaryotes.
Sec61 channel partners for cotranslational translocation
During cotranslational translocation, the Sec61 channel partners with the signal recognition particle (SRP), the signal recognition particle receptor (SR), and the ribosomes to transport the nascent polypeptide chain...
10.9K
Mechanism of Conjugation01:19

Mechanism of Conjugation

1.4K
Bacterial conjugation is a mechanism of horizontal gene transfer that enables the exchange of genetic material between bacterial cells through direct contact. This process is facilitated by a donor cell carrying a conjugative plasmid, which encodes genes necessary for pilus formation, DNA replication, and transfer. The conjugative plasmid plays a central role in initiating and executing the transfer of genetic material.The tra region of the conjugative plasmid encodes proteins responsible for...
1.4K
Gram-negative Bacterial Protein Secretion Systems01:17

Gram-negative Bacterial Protein Secretion Systems

1.4K
Gram-negative bacteria utilize sophisticated protein secretion systems to transport proteins across their double-membrane envelope into the extracellular environment or host cells. Based on their mechanism of action, these systems are classified into one-step and two-step pathways.One-Step Secretion Systems (Types I, III, IV, and VI)One-step secretion systems bypass the periplasm entirely, forming a continuous channel that spans both the inner and outer membranes:Type I Secretion System (T1SS):...
1.4K
Post-translational Translocation of Proteins to the RER01:27

Post-translational Translocation of Proteins to the RER

8.0K
A sizable fraction of proteins destined for ER are first synthesized in the cell cytosol and then transported across the ER membrane–a process called post-translational translocation. Similar to cotranslationally translocated proteins, these proteins also use the Sec translocon complex to enter the ER lumen.
Targeting proteins to the ER
Hsp40 and Hsp70 chaperone molecules bind the translated proteins in the cytosol to prevent their folding. The chaperone binding helps to keep the signal...
8.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Intercellular mitochondrial transfer and trans-mitophagy in response to protein import dysfunction.

The Journal of cell biology·2026
Same author

DOCKweb: a web-based GUI platform for molecular modeling with DOCK6.

Journal of computer-aided molecular design·2026
Same author

Transmembrane domain switching controls PINK1 import and fate in mitochondria.

The EMBO journal·2026
Same author

<i>In Silico</i> Discovery and Characterization of a Novel Nuclear Transcription Factor-Y (NF-Y) Inhibitor with Antimitogenic Properties.

Journal of medicinal chemistry·2026
Same author

The Great Escape: Protein Trafficking from the Bacterial Cytosol to the Outer Membrane.

Annual review of biochemistry·2026
Same author

Insights from aquaporin structures into drug-resistant sleeping sickness.

eLife·2026

Related Experiment Video

Updated: Mar 24, 2026

Creation of a Dense Transposon Insertion Library Using Bacterial Conjugation in Enterobacterial Strains Such As Escherichia Coli or Shigella flexneri
11:36

Creation of a Dense Transposon Insertion Library Using Bacterial Conjugation in Enterobacterial Strains Such As Escherichia Coli or Shigella flexneri

Published on: September 23, 2017

16.9K

Unlocking the Bacterial SecY Translocon

Robin A Corey1, William J Allen1, Joanna Komar1

  • 1School of Biochemistry, University of Bristol, University Walk, Bristol BS8 1TD, UK.

Structure (London, England : 1993)
|March 15, 2016
PubMed
Summary

The Sec translocon

More Related Videos

Conjugative Mating Assays for Sequence-specific Analysis of Transfer Proteins Involved in Bacterial Conjugation
10:41

Conjugative Mating Assays for Sequence-specific Analysis of Transfer Proteins Involved in Bacterial Conjugation

Published on: January 4, 2017

14.5K
Site-specific Bacterial Chromosome Engineering: &#934;C31 Integrase Mediated Cassette Exchange (IMCE)
08:21

Site-specific Bacterial Chromosome Engineering: ΦC31 Integrase Mediated Cassette Exchange (IMCE)

Published on: March 16, 2012

16.2K

Related Experiment Videos

Last Updated: Mar 24, 2026

Creation of a Dense Transposon Insertion Library Using Bacterial Conjugation in Enterobacterial Strains Such As Escherichia Coli or Shigella flexneri
11:36

Creation of a Dense Transposon Insertion Library Using Bacterial Conjugation in Enterobacterial Strains Such As Escherichia Coli or Shigella flexneri

Published on: September 23, 2017

16.9K
Conjugative Mating Assays for Sequence-specific Analysis of Transfer Proteins Involved in Bacterial Conjugation
10:41

Conjugative Mating Assays for Sequence-specific Analysis of Transfer Proteins Involved in Bacterial Conjugation

Published on: January 4, 2017

14.5K
Site-specific Bacterial Chromosome Engineering: &#934;C31 Integrase Mediated Cassette Exchange (IMCE)
08:21

Site-specific Bacterial Chromosome Engineering: ΦC31 Integrase Mediated Cassette Exchange (IMCE)

Published on: March 16, 2012

16.2K

Area of Science:

  • Molecular Biology
  • Cell Biology
  • Biochemistry

Background:

  • The Sec translocon (SecYEG in bacteria, Sec61 in eukaryotes) facilitates protein transport across membranes.
  • The precise mechanism of pre-protein translocation, particularly the role of ATPase SecA in bacteria, remains unclear despite structural data.

Purpose of the Study:

  • To investigate the impact of signal sequence binding on the SecYEG translocon.
  • To elucidate the dynamic conformational changes involved in initiating protein translocation.

Main Methods:

  • Biochemical analyses
  • Computational analyses
  • Exploration of signal sequence binding to SecYEG

Main Results:

  • Signal sequence binding induces a movement in transmembrane helix 7, unlocking the translocon.
  • This conformational change is transmitted to SecY and SecE on the cytoplasmic side, influencing SecA interaction.
  • The combined action of the signal sequence and SecA at the translocon's cytoplasmic face is critical for pre-protein insertion and translocation.

Conclusions:

  • The study reveals a dynamic mechanism for translocon activation initiated by signal sequence binding.
  • Conformational changes in SecYEG are key to communicating signals between the signal sequence and SecA.
  • This work advances the understanding of the initiation phase of protein translocation through the SecY channel.