Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Affinity Chromatography01:03

Affinity Chromatography

3.4K
Affinity chromatography is a powerful technique extensively utilized for separating and purifying specific biomolecules from complex mixtures. It capitalizes on the highly selective binding between an analyte and its counterpart, such as antibody-antigen interactions. The counterpart is immobilized on the stationary phase, forming an affinity column. The stationary phase typically consists of solid support, such as agarose or porous glass beads, immobilizing the affinity ligand. The mobile...
3.4K
Peptide Identification Using Tandem Mass Spectrometry01:33

Peptide Identification Using Tandem Mass Spectrometry

8.8K
Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
8.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Synthesis and machine learning techniques to enable data-driven investigation of supramolecular host-guest interactions.

Chemical science·2026
Same author

Efficacy and safety evaluation of artificial intelligence-identified antimicrobial peptides targeting avian pathogenic Escherichia coli in broiler chickens.

Journal of animal science and biotechnology·2026
Same author

Tetrachloroanthracene Walled Glycoluril Dimer Undergoes Self-Association and 1:1, 2:2, and 1:3 Host•Guest Binding.

Supramolecular chemistry·2026
Same author

Systematic Evaluation of Maleimide Spacer Impact on Drug-Linker Deconjugation in Antibody-Drug Conjugates <i>Ex Vivo</i> and <i>In Vivo</i>.

Bioconjugate chemistry·2026
Same author

Information-rich sensors by assembly: a review on the use of macrocyclic hosts in the context of complex chemical systems.

Chemical communications (Cambridge, England)·2025
Same author

Pharmacological targeting of CBX7 alters the epigenetic landscape and induces differentiation of leukemic cells.

Blood neoplasia·2025
Same journal

Modeling the Effects of Short-Range Randomness in Packed Sphere Beds.

Analytical chemistry·2026
Same journal

Mitochondrial Redox Cascade-Directed Covalent NIR Fluorogenic Imaging of Therapy-Induced Senescence Integrates Tumor and Host Responses.

Analytical chemistry·2026
Same journal

Proteomic Profiling of RHD-Related Mitral Annulus Calcification Enabled by Magnetic Carbon Nanomaterial-Supported Quasi-Immobilized Enzyme Digestion.

Analytical chemistry·2026
Same journal

Spatial-Photonic Encoding on a Single Fiber: Breaking the Bottleneck in Photoelectrochemical Biosensing for Precision Diagnostics.

Analytical chemistry·2026
Same journal

Spreadable Biosensing Pregel for Analyte Visualization in Peeled Plant Tissues.

Analytical chemistry·2026
Same journal

DARibo-Q: RNA Allosteric Transduction for Fluorescence Imaging of Dopamine Modulation in Living Systems.

Analytical chemistry·2026
See all related articles

Related Experiment Video

Updated: Mar 24, 2026

A Mass Spectrometry-Based Proteomics Approach for Global and High-Confidence Protein R-Methylation Analysis
09:40

A Mass Spectrometry-Based Proteomics Approach for Global and High-Confidence Protein R-Methylation Analysis

Published on: April 28, 2022

3.1K

Supramolecular Affinity Chromatography for Methylation-Targeted Proteomics.

Graham A E Garnett1, Melissa J Starke1, Alok Shaurya1

  • 1Department of Chemistry, University of Victoria , Victoria, British Columbia V8W3 V6, Canada.

Analytical Chemistry
|March 15, 2016
PubMed
Summary
This summary is machine-generated.

Researchers developed a novel methyllysine-affinity column for improved methylation proteomics. This chemical approach enhances the analysis of methyllysine residues in complex protein samples, overcoming limitations of current antibody-based methods.

More Related Videos

Profiling of Methyltransferases and Other S-adenosyl-L-homocysteine-binding Proteins by Capture Compound Mass Spectrometry CCMS
17:12

Profiling of Methyltransferases and Other S-adenosyl-L-homocysteine-binding Proteins by Capture Compound Mass Spectrometry CCMS

Published on: December 20, 2010

16.1K
Specificity Analysis of Protein Lysine Methyltransferases Using SPOT Peptide Arrays
08:48

Specificity Analysis of Protein Lysine Methyltransferases Using SPOT Peptide Arrays

Published on: November 29, 2014

14.5K

Related Experiment Videos

Last Updated: Mar 24, 2026

A Mass Spectrometry-Based Proteomics Approach for Global and High-Confidence Protein R-Methylation Analysis
09:40

A Mass Spectrometry-Based Proteomics Approach for Global and High-Confidence Protein R-Methylation Analysis

Published on: April 28, 2022

3.1K
Profiling of Methyltransferases and Other S-adenosyl-L-homocysteine-binding Proteins by Capture Compound Mass Spectrometry CCMS
17:12

Profiling of Methyltransferases and Other S-adenosyl-L-homocysteine-binding Proteins by Capture Compound Mass Spectrometry CCMS

Published on: December 20, 2010

16.1K
Specificity Analysis of Protein Lysine Methyltransferases Using SPOT Peptide Arrays
08:48

Specificity Analysis of Protein Lysine Methyltransferases Using SPOT Peptide Arrays

Published on: November 29, 2014

14.5K

Area of Science:

  • Proteomics
  • Chemical Biology
  • Biochemistry

Background:

  • Proteome-wide studies of post-translational modifications like methylation are crucial but face challenges.
  • Current antibody-based enrichment methods for methyllysine peptides lack reliability and standardization.
  • Mass spectrometry analysis is complicated by sample diversity and ionization competition.

Purpose of the Study:

  • To develop a novel, reliable chemical affinity method for enriching methyllysine-containing peptides.
  • To overcome the limitations of existing antibody-based enrichment techniques in methylation proteomics.
  • To improve the analysis of methyllysine residues in complex biological samples.

Main Methods:

  • Invented an anionic supramolecular host for selective methyllysine-peptide complexation.
  • Developed a methyllysine-affinity chromatography column using the supramolecular host.
  • Separated a proteolyzed nuclear extract on the methyl-affinity column prior to mass spectrometry analysis.

Main Results:

  • The methyllysine-affinity column selectively resolves peptides based on methylation status.
  • This method achieved peptide separation impossible with standard cation-exchange columns.
  • Enrichment and improved analysis of methyllysine residues from complex protein mixtures were demonstrated.

Conclusions:

  • Chemical methyl-affinity columns represent a significant advance for enrichment and analysis in proteomics.
  • This novel approach offers a standardized and reliable platform for methylation studies.
  • The findings highlight the potential of biomolecule-driven enrichment methods in complex biological analyses.