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A Mouse Model of Intestinal Partial Obstruction

A Mouse Model of Intestinal Partial Obstruction

Published on: March 5, 2018

Development of a novel mouse constipation model.

Chao Liang¹, Kai-Yue Wang¹, Zhi Yu¹

¹Chao Liang, Kai-Yue Wang, Zhi Yu, Bin Xu, Key Laboratory of Integrated Acupuncture and Drugs Affiliated to the Ministry of Education of China, Nanjing University of Chinese Medicine, Nanjing 210023, Jiangsu Province, China.

World Journal of Gastroenterology

|March 15, 2016

Summary

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Ice-cold saline successfully induced constipation in mice, characterized by reduced stool weight and altered form. This method provides a reliable model for studying constipation pathogenesis and potential treatments.

Area of Science:

Gastroenterology
Animal Models
Physiology

Background:

Constipation is a prevalent gastrointestinal disorder with significant impact on quality of life.
Developing reliable animal models is crucial for understanding constipation's underlying mechanisms and testing therapeutic interventions.

Purpose of the Study:

To establish and validate a novel mouse model of constipation using ice-cold saline.
To assess the efficacy of ice-cold saline in inducing consistent and measurable constipation symptoms.

Main Methods:

Mice were administered ice-cold saline (0-4°C) or control saline (15-20°C) intragastrically for 14 days.
Stool characteristics (weight, form) and intestinal propulsion were evaluated.
Immunohistochemistry was used to detect neuronal marker PGP9.5 expression.

Keywords:

Cold water Constipation ENS Mouse model

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Main Results:

Ice-cold saline administration led to decreased stool wet weight (15%-25%) and smaller, drier stools in a subset of mice (State III).
Constipated mice exhibited degenerated intestinal function and significantly reduced PGP9.5 expression in the myenteric plexus.
Stool weight changes served as a reliable indicator for classifying constipation severity.

Conclusions:

Intragastric ice-cold saline is a stable and repeatable method for creating a mouse constipation model.
This model is suitable for investigating constipation's pathogenesis and evaluating new treatment strategies.
Changes in stool weight and size are valuable metrics for confirming successful constipation induction in this model.