Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

2.8K
Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
2.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

External electric field-induced melt deformations in laser-directed powder processing.

Optics letters·2026
Same author

Influence of Precursor Composition on Microstructure Formation in Protein-Derived Porous Graphitic Aerogels.

ACS materials Au·2026
Same author

Reactive Laser Additive Manufacturing of Hierarchically Structured Aerogels.

Advanced materials (Deerfield Beach, Fla.)·2026
Same author

Self-Templated Hierarchically Porous Graphitic Aerogels for Emi Shielding.

Small (Weinheim an der Bergstrasse, Germany)·2026
Same author

Multi-Scale Structural Effects of External Electrical Fields of Melt Tracks in Laser Powder Bed Fusion.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026
Same author

C<sub>60</sub> Fullerene as an On-Demand Single Photon Source at Room Temperature.

Nano letters·2025

Related Experiment Video

Updated: Mar 24, 2026

A Protocol for Real-time 3D Single Particle Tracking
10:16

A Protocol for Real-time 3D Single Particle Tracking

Published on: January 3, 2018

15.4K

Three-dimensional particle tracking via tunable color-encoded multiplexing.

Martí Duocastella, Christian Theriault, Craig B Arnold

    Optics Letters
    |March 15, 2016
    PubMed
    Summary
    This summary is machine-generated.

    This study introduces a new 3D particle tracking method using a specialized microscope lens. It achieves nanometric precision over extended ranges, improving particle localization and visualization.

    More Related Videos

    Three-dimensional Particle Tracking Velocimetry for Turbulence Applications: Case of a Jet Flow
    13:02

    Three-dimensional Particle Tracking Velocimetry for Turbulence Applications: Case of a Jet Flow

    Published on: February 27, 2016

    13.2K
    Multi-color Localization Microscopy of Single Membrane Proteins in Organelles of Live Mammalian Cells
    11:06

    Multi-color Localization Microscopy of Single Membrane Proteins in Organelles of Live Mammalian Cells

    Published on: June 30, 2018

    9.2K

    Related Experiment Videos

    Last Updated: Mar 24, 2026

    A Protocol for Real-time 3D Single Particle Tracking
    10:16

    A Protocol for Real-time 3D Single Particle Tracking

    Published on: January 3, 2018

    15.4K
    Three-dimensional Particle Tracking Velocimetry for Turbulence Applications: Case of a Jet Flow
    13:02

    Three-dimensional Particle Tracking Velocimetry for Turbulence Applications: Case of a Jet Flow

    Published on: February 27, 2016

    13.2K
    Multi-color Localization Microscopy of Single Membrane Proteins in Organelles of Live Mammalian Cells
    11:06

    Multi-color Localization Microscopy of Single Membrane Proteins in Organelles of Live Mammalian Cells

    Published on: June 30, 2018

    9.2K

    Area of Science:

    • Optical microscopy
    • Particle tracking
    • Nanotechnology

    Background:

    • Accurate 3D particle localization is crucial for various scientific fields.
    • Conventional methods often face limitations in axial range and precision.
    • The coffee-stain phenomenon involves complex particle dynamics during droplet evaporation.

    Purpose of the Study:

    • To develop a novel 3D tracking approach for high-precision particle localization.
    • To extend the axial tracking range beyond conventional limits.
    • To visualize dynamic phenomena like droplet evaporation using the developed method.

    Main Methods:

    • Implementation of a fast acousto-optic liquid lens in a bright field microscope.
    • Multiplexing light into selectable focal planes using color channels (red, green, blue).
    • Retrieving multiplane information from particle diffraction rings for precise localization.

    Main Results:

    • Achieved nanometric precision in 3D particle tracking.
    • Extended the axial tracking range approximately fivefold compared to single-plane methods.
    • Successfully applied the method to visualize the 3D dynamics of the coffee-stain phenomenon.

    Conclusions:

    • The novel acousto-optic liquid lens enables enhanced 3D particle tracking.
    • This technique offers significant improvements in precision and axial range for microscopy.
    • The method provides new insights into dynamic processes like droplet evaporation.