Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Proteomics01:33

Proteomics

10.1K
A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...
10.1K
X-ray Crystallography02:18

X-ray Crystallography

26.7K
The size of the unit cell and the arrangement of atoms in a crystal may be determined from measurements of the diffraction of X-rays by the crystal, termed X-ray crystallography.
Diffraction
Diffraction is the change in the direction of travel experienced by an electromagnetic wave when it encounters a physical barrier whose dimensions are comparable to those of the wavelength of the light. X-rays are electromagnetic radiation with wavelengths about as long as the distance between neighboring...
26.7K
Determination of Crystal Structures01:29

Determination of Crystal Structures

58
In the late 1800s, the revelation that light extended beyond visible wavelengths led to the discovery of X-rays by Wilhelm Roentgen. Recognized as high-energy electromagnetic radiation with short wavelengths, X-rays prompted exploration into their interaction with crystals. Max von Laue proposed in 1912 that the periodic arrangement of atoms, ions, or molecules in crystals would cause them to diffract X-rays, a hypothesis confirmed through experiments with copper sulfate and zinc sulfide...
58

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Nature's Anaerobic Toolkit: Glycyl Radical Enzymes and Their Expanding Functional and Mechanistic Diversity.

Biochemistry·2026
Same author

The Structural Basis of Malodorant Skatole Formation by the Glycyl Radical Enzyme Indoleacetate Decarboxylase.

bioRxiv : the preprint server for biology·2026
Same author

LEGO®-inspired electrically-actuated microfluidics for on-chip protein crystallization and <i>in situ</i> X-ray crystallography.

Lab on a chip·2026
Same author

How a protein repurposes vitamin B12 as a light sensor.

Nature·2026
Same author

A highly dynamic mononuclear non-heme iron enzyme for the two-step isonitrile biosynthesis.

Nature communications·2026
Same author

The cobalamin-binding domain of cobalamin-dependent radical S-adenosylmethionine enzymes: Familiarity in unfamiliar places.

Journal of inorganic biochemistry·2026
Same journal

Design Principles for Negative Thermal Expansion in Two-Dimensional Materials.

Accounts of chemical research·2026
Same journal

Main Group Redox Catalysis: New Frontiers with Germanium and Tin.

Accounts of chemical research·2026
Same journal

Taming Irreversibility in sp<sup>2</sup>-Carbon-Conjugated COFs from Polycrystalline Powders to Single Crystals and Thin Films.

Accounts of chemical research·2026
Same journal

Electroactive Imidazolium Ionic Liquids in Organic Synthesis.

Accounts of chemical research·2026
Same journal

Calix[4]resorcinarene-Based Porous Organic Cages: Synthesis and Applications.

Accounts of chemical research·2026
Same journal

Light-Driven Dual Rotary Molecular Motors and Beyond.

Accounts of chemical research·2026
See all related articles

Related Experiment Video

Updated: Mar 24, 2026

Microcrystallography of Protein Crystals and In Cellulo Diffraction
09:35

Microcrystallography of Protein Crystals and In Cellulo Diffraction

Published on: July 21, 2017

9.6K

Metalloprotein Crystallography: More than a Structure.

Sarah E J Bowman1, Jennifer Bridwell-Rabb1, Catherine L Drennan1

  • 1Department of Chemistry, ‡Department of Biology, and §Howard Hughes Medical Institute, Massachusetts Institute of Technology , 77 Massachusetts Avenue, Cambridge, Massachusetts 02139, United States.

Accounts of Chemical Research
|March 16, 2016
PubMed
Summary
This summary is machine-generated.

Metalloproteins utilize metal ions for essential functions. Their unique properties aid in structural determination through X-ray crystallography, enabling advanced biochemical insights.

More Related Videos

Neutron Crystallography Data Collection and Processing for Modelling Hydrogen Atoms in Protein Structures
10:10

Neutron Crystallography Data Collection and Processing for Modelling Hydrogen Atoms in Protein Structures

Published on: December 1, 2020

5.8K
Harvesting and Cryo-cooling Crystals of Membrane Proteins Grown in Lipidic Mesophases for Structure Determination by Macromolecular Crystallography
18:45

Harvesting and Cryo-cooling Crystals of Membrane Proteins Grown in Lipidic Mesophases for Structure Determination by Macromolecular Crystallography

Published on: September 2, 2012

25.8K

Related Experiment Videos

Last Updated: Mar 24, 2026

Microcrystallography of Protein Crystals and In Cellulo Diffraction
09:35

Microcrystallography of Protein Crystals and In Cellulo Diffraction

Published on: July 21, 2017

9.6K
Neutron Crystallography Data Collection and Processing for Modelling Hydrogen Atoms in Protein Structures
10:10

Neutron Crystallography Data Collection and Processing for Modelling Hydrogen Atoms in Protein Structures

Published on: December 1, 2020

5.8K
Harvesting and Cryo-cooling Crystals of Membrane Proteins Grown in Lipidic Mesophases for Structure Determination by Macromolecular Crystallography
18:45

Harvesting and Cryo-cooling Crystals of Membrane Proteins Grown in Lipidic Mesophases for Structure Determination by Macromolecular Crystallography

Published on: September 2, 2012

25.8K

Area of Science:

  • Biochemistry and structural biology
  • Metalloprotein research
  • X-ray crystallography

Background:

  • Metal ions and metallocofactors are crucial for numerous biochemical reactions, with 25-50% of the proteome relying on them.
  • Metalloproteins' diverse functions stem from metals' variable redox states and geometries, enhanced by organic frameworks in metallocofactors.
  • Structural characterization of metalloproteins is key for understanding their enzymatic mechanisms.

Purpose of the Study:

  • To highlight the advantageous uses of metals in metalloprotein crystallography.
  • To demonstrate how metals and metallocofactors facilitate structure solution and functional analysis.
  • To showcase advancements in metalloprotein structure determination using synchrotron techniques.

Main Methods:

  • Utilizing metallocofactors for phasing information in X-ray crystallography.
  • Employing K-edge X-ray absorption spectroscopy (XAS) for metal identification in crystals.
  • Applying UV-vis spectroscopy on crystals to assess protein enzymatic activity.

Main Results:

  • Metals provide unique signatures for anomalous dispersion, aiding phase determination in crystallography.
  • K-edge XAS and UV-vis spectroscopy offer powerful, non-destructive analytical tools for metalloprotein crystals.
  • Advancements in synchrotron technology enhance the capabilities for metalloprotein structure determination.

Conclusions:

  • Metals and metallocofactors offer significant advantages for metalloprotein structure determination and functional studies.
  • Integrated spectroscopic techniques at synchrotrons provide comprehensive insights into metalloprotein function.
  • This approach advances our understanding of metalloproteins' roles in biological systems.