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Updated: Mar 24, 2026

Direct Imaging of Laser-driven Ultrafast Molecular Rotation

Direct Imaging of Laser-driven Ultrafast Molecular Rotation

Published on: February 4, 2017

Functional second harmonic generation microscopy probes molecular dynamics with high temporal resolution.

Moritz Förderer¹, Tihomir Georgiev², Matias Mosqueira²

¹Medical Biophysics Unit, Institute for Physiology and Pathophysiology, University of Heidelberg, Heidelberg, Germany; m.foerderer@physiologie.uni-heidelberg.de.

Biomedical Optics Express

|March 16, 2016

Summary

This summary is machine-generated.

This study introduces high-speed functional second harmonic generation (SHG) microscopy for dynamic molecular imaging. The new method achieves millisecond temporal resolution, offering insights into motor protein myosin dynamics in muscle cells.

Keywords:

(170.2655) Functional monitoring and imaging (180.4315) Nonlinear microscopy (190.2620) Harmonic generation and mixing

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Area of Science:

Biophysics
Cell Biology
Microscopy

Background:

Second harmonic generation (SHG) microscopy enables label-free imaging of endogenous proteins like myosin and collagen.
Polarization-resolved SHG microscopy provides additional molecular state information.
There is a need for high-speed functional imaging methods to study dynamic biological processes.

Purpose of the Study:

To develop and demonstrate high-speed functional SHG microscopy techniques.
To investigate the molecular dynamics of myosin in mammalian muscle cells with millisecond resolution.

Main Methods:

Development of two novel approaches using linearly polarized light for SHG microscopy.
Implementation of high-speed line scan measurements.
Application to mammalian muscle cells to observe motor protein dynamics.

Main Results:

Demonstrated high-speed line scan measurements of molecular dynamics.
Achieved a time resolution of 1 millisecond for imaging myosin.
Successfully applied the technique to mammalian muscle cells.

Conclusions:

High-speed functional SHG microscopy offers a powerful tool for studying dynamic molecular processes.
The developed methods provide new insights into the structural and temporal dynamics of proteins in biological systems.
This technique has significant potential for ex vivo and in vivo investigations.