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Isolation of Human Myoblasts, Assessment of Myogenic Differentiation, and Store-operated Calcium Entry Measurement
Published on: July 26, 2017
Direct organelle thermometry with fluorescence lifetime imaging microscopy in single myotubes
Hideki Itoh1, Satoshi Arai2, Thankiah Sudhaharan3
1Department of Pure and Applied Physics, Graduate School of Advanced Science and Engineering, Waseda University, Tokyo 169-8555, Japan and Institute of Medical Biology, Agency for Science, Technology and Research (A*STAR), Singapore 138648, Singapore. birgit.lane@imb.a-star.edu.sg.
Abstract:
We describe organelle thermometry using an endoplasmic reticulum-targeting small molecule dye and cytosolic mCherry, whose fluorescence lifetimes reduce with increasing temperature and can be monitored by fluorescence lifetime imaging microscopy. The results show that heat production in single myotubes is highly localized and is coupled to a Ca(2+) burst.
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