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A Method for Selecting Structure-switching Aptamers Applied to a Colorimetric Gold Nanoparticle Assay
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SELEX Modifications and Bioanalytical Techniques for Aptamer-Target Binding Characterization.

Sze Y Tan1,2, Caleb Acquah1,2, Amandeep Sidhu2,3

  • 1a Department of Chemical Engineering , Curtin University , Sarawak , Malaysia.

Critical Reviews in Analytical Chemistry
|March 17, 2016
PubMed
Summary

Aptamers, or synthetic DNA/RNA molecules, are key for detecting biomolecules. This review covers aptamer generation, binding characteristics, and applications, highlighting advancements in SELEX technology.

Keywords:
AptamerSELEXbiomoleculesbiophysical techniquescellular targets

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Area of Science:

  • Biotechnology
  • Molecular Biology
  • Analytical Chemistry

Background:

  • Affinity bioprobes are crucial for detecting biomolecules and cells in life sciences.
  • Aptamers, generated via Systematic Evolution of Ligands by Exponential Enrichment (SELEX), are highly specific affinity probes.

Purpose of the Study:

  • To review advancements in aptamer generation and SELEX protocols.
  • To analyze factors influencing aptamer binding to cellular and biomolecular targets.
  • To discuss the applications of aptameric binding and characterization techniques.

Main Methods:

  • Review of literature on aptamer generation and SELEX.
  • Analysis of physicochemical conditions affecting aptamer-target interactions.
  • Discussion of biophysical techniques for aptamer characterization.

Main Results:

  • Modified SELEX protocols yield highly specific and stable aptamers.
  • Various techniques exist for analyzing aptamer-target binding, each with pros and cons.
  • Aptamers have diverse field applications in biomolecular detection.

Conclusions:

  • Aptamer technology continues to advance, offering powerful tools for life sciences.
  • Understanding binding characteristics and employing appropriate characterization methods are essential for aptamer utility.
  • Further research in aptamer generation and application holds significant promise.