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Spleen tyrosine kinase induces MUC5AC expression in human airway epithelial cell

Hyung Gyun Na1, Chang Hoon Bae, Yoon Seok Choi

  • 1Department of Otorhinolaryngology-Head and Neck Surgery, College of Medicine, Yeungnam University, Daegu, Republic of Korea.

American Journal of Rhinology & Allergy
|March 17, 2016
PubMed
Abstract

Insights

Spleen tyrosine kinase (SYK) increases MUC5AC expression in airway cells. This occurs through the activation of extracellular signal-related kinase (ERK) and p38 mitogen-activated protein kinase (MAPK) pathways, suggesting SYK as a therapeutic target.

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Immunology

Background:

  • MUC5AC mucin is elevated in chronic airway inflammation.
  • Spleen tyrosine kinase (SYK) regulates inflammatory signaling.
  • SYK expression is found in respiratory epithelial cells, but its role in mucin secretion is unknown.

Purpose of the Study:

  • To investigate the effect of SYK on MUC5AC expression in human airway epithelial cells.
  • To elucidate the signaling pathways involved in SYK-mediated MUC5AC regulation.

Main Methods:

  • Utilized NCI-H292 cells and primary human nasal epithelial cells.
  • Assessed MUC5AC expression using RT-PCR, real-time PCR, ELISA, and Western blot.
  • Employed specific inhibitors (U0126, SB203580) and siRNA for SYK, ERK, and p38 MAPK.

Main Results:

  • SYK significantly induced MUC5AC expression.
  • SYK activated ERK1/2 and p38 MAPK signaling pathways.
  • Inhibition or knockdown of ERK and p38 MAPK attenuated SYK-induced MUC5AC expression.

Conclusions:

  • SYK enhances MUC5AC expression in human airway epithelial cells.
  • The ERK and p38 MAPK pathways mediate SYK's effect on MUC5AC.
  • SYK is a key regulator of MUC5AC production in airway inflammation.

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