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Assessment of Cellular Oxidation using a Subcellular Compartment-Specific Redox-Sensitive Green Fluorescent Protein

Assessment of Cellular Oxidation using a Subcellular Compartment-Specific Redox-Sensitive Green Fluorescent Protein

Published on: June 18, 2020

Mouse redox histology using genetically encoded probes.

Yuuta Fujikawa¹, Leticia P Roma¹, Mirko C Sobotta¹

¹Division of Redox Regulation, DKFZ-ZMBH (German Cancer Research Center-Center for Molecular Biology of the University of Heidelberg) Alliance, DKFZ, Im Neuenheimer Feld 280, 69120 Heidelberg, Germany.

Science Signaling

|March 17, 2016

Summary

This summary is machine-generated.

Researchers developed a new method to map the in vivo distribution of oxidants in animal tissues. This technique visualizes redox state changes in various biological contexts, aiding biomedical research.

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Area of Science:

Biomedical research
Cellular biology
Physiology

Background:

Mapping in vivo oxidant distribution in animal tissues is crucial for understanding health and disease.
Redox changes are influenced by various factors like diet, aging, and toxins.
Current tools lack the precision to pinpoint redox state changes at organ, tissue, cell, or subcellular levels.

Purpose of the Study:

To develop and demonstrate a novel procedure for preserving and visualizing the in vivo redox state of genetically encoded biosensors in histological tissue sections.
To create detailed "redox maps" of tissues for comparative analysis.
To showcase the technique's utility in diverse biological scenarios.

Main Methods:

Utilizing genetically encoded redox biosensors.
Preserving the in vivo redox state within histological tissue sections.
Developing a procedure for high-resolution redox mapping.

Main Results:

Successfully visualized endogenous redox differences and changes in various biological contexts.
Demonstrated the technique's effectiveness in tumor growth, inflammation, embryonic development, and nutrient starvation models.
Generated "redox maps" providing unprecedented spatial resolution of oxidant distribution.

Conclusions:

The described procedure effectively preserves and visualizes in vivo redox states in histological sections.
This technique provides valuable "redox maps" for studying physiological and pathological processes.
The method offers a powerful new tool for biomedical research requiring precise redox state analysis.