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Profiling the human immune response to Mycobacterium tuberculosis by human cytokine array
Tao Chen1, Zhenyan Li2, Li Yu3
1Center for Tuberculosis Control of Guangdong Province, Guangzhou 510630, China.
Tuberculosis (Edinburgh, Scotland)
|March 17, 2016
Summary
Serum cytokines show promise as biomarkers for tuberculosis (TB). Specific cytokines like I-309 and MIG, along with cytokine ratios, can help screen for latent tuberculosis infection (LTBI) and active TB.
Area of Science:
- Immunology
- Infectious Diseases
- Biomarker Discovery
Background:
- Tuberculosis (TB) remains a global health threat with limited rapid diagnostic biomarkers.
- Accurate screening for latent TB infection (LTBI) and active TB is crucial for effective management.
Purpose of the Study:
- To identify and evaluate serum cytokines as potential biomarkers for diagnosing LTBI and active TB.
- To assess the diagnostic efficacy of individual cytokines and their combinations.
Main Methods:
- Cytokine array analysis to profile dynamic changes post-TB infection.
- Enzyme-linked immunosorbent assay (ELISA) to confirm differential cytokine expression.
- Receiver Operating Characteristic (ROC) curve analysis to determine diagnostic accuracy.
Main Results:
- Elevated levels of Eotaxin-2, ICAM-1, MCSF, IL-12p70, and IL-11 observed in LTBI individuals.
- Increased concentrations of I-309, MIG, Eotaxin-2, IL-8, ICAM-1, IL-6sR, and Eotaxin in active TB patients.
- High diagnostic accuracy (AUCs up to 0.898) for I-309, MIG, IL-8, and a combined cytokine panel in active TB detection.
Conclusions:
- Serum cytokines such as I-309, MIG, IL-8, Eotaxin-2, and ICAM-1 are potential biomarkers for LTBI and active TB.
- Cytokine combinations, including IFN-γ/IL-4 and IL-2/TNF-α ratios, demonstrate clinical utility for TB screening.
- These findings suggest promising applications for serum cytokines in the clinical diagnosis of TB.
