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Induced Pluripotent Stem Cells

Induced Pluripotent Stem Cells

Stem cells are undifferentiated cells that divide and produce different types of cells. Ordinarily, cells that have differentiated into a specific cell type are post-mitotic—that is, they no longer divide. However, scientists have found a way to reprogram these mature cells so that they “de-differentiate” and return to an unspecialized, proliferative state. These cells are also pluripotent like embryonic stem cells—able to produce all cell types—and are therefore...

Induced Pluripotent Stem Cells

Induced Pluripotent Stem Cells

Stem cells are undifferentiated cells that divide and produce different cell types. Ordinarily, cells that have differentiated into a specific cell type are terminally differentiated; however, scientists have found a way to reprogram these mature cells so that they dedifferentiate and return to an unspecialized, proliferative state. These cells are pluripotent like embryonic stem cells—able to produce all cell types—and are called induced pluripotent stem cells (iPSCs).
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Related Experiment Video

Updated: Mar 24, 2026

Generation of Mice Derived from Induced Pluripotent Stem Cells

Generation of Mice Derived from Induced Pluripotent Stem Cells

Published on: November 29, 2012

Germline Competent Pluripotent Mouse Stem Cells Generated by Plasmid Vectors.

Chien-Hong Chen¹, Yu-Hsiu Su¹, Kun-Hsiung Lee²

¹a Division of Animal Technology, Laboratories of Animal Technology , Agricultural Technology Research Institute , Hsinchu City , Taiwan.

Animal Biotechnology

|March 17, 2016

Summary

Researchers developed new methods for reprogramming mouse cells into induced pluripotent stem cells (iPSCs). A six-factor method significantly improved reprogramming efficiency, yielding germline-competent iPSC clones.

Keywords:

Germline transmission iPSC plasmid

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Last Updated: Mar 24, 2026

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Generation of Human Induced Pluripotent Stem Cells from Peripheral Blood Using the STEMCCA Lentiviral Vector

Generation of Human Induced Pluripotent Stem Cells from Peripheral Blood Using the STEMCCA Lentiviral Vector

Published on: October 31, 2012

Area of Science:

Stem cell biology
Molecular biology
Genetics

Background:

Induced pluripotent stem cells (iPSCs) hold promise for regenerative medicine and disease modeling.
Efficient and reliable methods for generating iPSCs are crucial for their clinical application.

Purpose of the Study:

To develop non-integrated methods for reprogramming mouse embryonic fibroblast (MEF) cells into iPSCs.
To enhance the efficiency of iPSC generation using additional reprogramming factors.

Main Methods:

Utilized a combination of pig (pOct4, pSox2, pc-Myc) and human (hKLF4, hAID, hTDG) reprogramming factors delivered via plasmid vectors.
Compared a four-factor (4F) method with a six-factor (6F) method incorporating hAID and hTDG to assess reprogramming efficiency.

Main Results:

The 4F method successfully generated iPSC clones with naive embryonic stem cell (ESC)-like morphology, expressing endogenous Nanog and capable of chimera generation.
The 6F method increased reprogramming conversion efficiency approximately five-fold compared to the 4F method.
One of the 6F plasmid-derived iPSC (piPSC) clones demonstrated germline transmission competence.

Conclusions:

Non-integrated reprogramming using plasmid vectors is an effective strategy for generating iPSCs.
The addition of hAID and hTDG to the standard four factors significantly enhances iPSC reprogramming efficiency.
The developed 6F method provides a more efficient route to generate germline-competent iPSCs.