Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

SLFN11 binds DDX1 to counteract human mesenchymal stem/stromal cell senescence through inhibition of mTOR/eIF4E signaling pathway activation.

Life sciences·2026
Same author

Development and In Vitro Evaluation of Gefitinib-Salicylic Acid Nanocrystals for Improved Bioavailability.

Pharmaceutics·2026
Same author

<i>Saxifraga zhiminiae</i> (Saxifragaceae), a new species from Xizang, China.

PhytoKeys·2026
Same author

Pachymic acid targets PDIA6 to delay mesenchymal stem cell senescence through the G6PD/STAT3 signaling axis and prevent senile osteoporosis.

Journal of advanced research·2026
Same author

A Trifunctional Imine Reductase Enables a Three-Step Biocatalytic Cascade.

Chembiochem : a European journal of chemical biology·2026
Same author

1-NP hijacks endocrine-metabolic checkpoints and disrupts testicular steroidogenesis by suppressing the cAMP-PKA-CREB-HMGCR axis.

Toxicology letters·2025

Related Experiment Video

Updated: Mar 24, 2026

Development of Targeting Induced Local Lesions IN Genomes TILLING Populations in Small Grain Crops by Ethyl Methanesulfonate Mutagenesis
08:36

Development of Targeting Induced Local Lesions IN Genomes TILLING Populations in Small Grain Crops by Ethyl Methanesulfonate Mutagenesis

Published on: July 16, 2019

12.4K

Transcriptome Analysis for Abnormal Spike Development of the Wheat Mutant dms.

Xin-Xin Zhu1, Qiao-Yun Li1, Chun-Cai Shen1

  • 1National Centre of Engineering and Technological Research for Wheat, Henan Agricultural University / Key Laboratory of Physiological Ecology and Genetic Improvement of Food Crops in Henan Province, Zhengzhou, Henan, China.

Plos One
|March 17, 2016
PubMed
Summary

Dwarf wheat mutants (dms) exhibit abnormal spike development due to lower expression of homeotic genes and repressed biological processes. This study identifies key genes involved in wheat floral organ differentiation, offering insights into yield potential.

More Related Videos

Author Spotlight: Streamlining Rice Breeding with CRISPR/Cas for Obtaining Optimal Phenotypic and Agronomic Traits
09:43

Author Spotlight: Streamlining Rice Breeding with CRISPR/Cas for Obtaining Optimal Phenotypic and Agronomic Traits

Published on: January 3, 2025

3.6K
Obtaining High-Quality Transcriptome Data from Cereal Seeds by a Modified Method for Gene Expression Profiling
07:18

Obtaining High-Quality Transcriptome Data from Cereal Seeds by a Modified Method for Gene Expression Profiling

Published on: May 21, 2020

8.0K

Related Experiment Videos

Last Updated: Mar 24, 2026

Development of Targeting Induced Local Lesions IN Genomes TILLING Populations in Small Grain Crops by Ethyl Methanesulfonate Mutagenesis
08:36

Development of Targeting Induced Local Lesions IN Genomes TILLING Populations in Small Grain Crops by Ethyl Methanesulfonate Mutagenesis

Published on: July 16, 2019

12.4K
Author Spotlight: Streamlining Rice Breeding with CRISPR/Cas for Obtaining Optimal Phenotypic and Agronomic Traits
09:43

Author Spotlight: Streamlining Rice Breeding with CRISPR/Cas for Obtaining Optimal Phenotypic and Agronomic Traits

Published on: January 3, 2025

3.6K
Obtaining High-Quality Transcriptome Data from Cereal Seeds by a Modified Method for Gene Expression Profiling
07:18

Obtaining High-Quality Transcriptome Data from Cereal Seeds by a Modified Method for Gene Expression Profiling

Published on: May 21, 2020

8.0K

Area of Science:

  • Plant genetics and molecular biology
  • Crop science and breeding
  • Developmental biology

Background:

  • Wheat (Triticum aestivum L.) spike development is crucial for grain yield.
  • A novel dwarf, multi-pistil, and sterile mutant (dms) was identified, with dwarf (D) and tall (T) genotypes suitable for studying spike development.
  • The molecular basis for the spike abnormalities in the dwarf genotype requires investigation.

Purpose of the Study:

  • To explore the molecular mechanisms underlying spike abnormalities in the dwarf genotype of the dms wheat mutant.
  • To identify genes and biological processes affected in dwarf wheat spikes compared to tall counterparts.

Main Methods:

  • RNA sequencing was performed on super-bulked differentiating spikes and stem tips from dwarf (D) and tall (T) wheat plants.
  • Integrative analysis was used to identify differentially expressed genes and highly expressed genes in spikes.
  • Gene expression levels were compared between D and T genotypes.

Main Results:

  • Four unigene libraries were constructed, identifying 419 highly expressed genes in spikes, including MADS-box family homeotic genes (e.g., TaAP2, TaFL, TaDL).
  • 143 genes showed significant differential expression between young spikes of T and D plants.
  • Expression levels of genes involved in transcription, translation, cell division, photosynthesis, and metabolism were significantly lower in D than in T plants.

Conclusions:

  • A set of genes related to wheat floral organ differentiation, including homeotic genes, were identified.
  • Lower expression of homeotic genes, hormonal imbalance, repressed biological processes, and deficiencies in materials/energy contribute to spike abnormalities in dms mutants.
  • Further research is needed to fully elucidate the causal factors, particularly hormonal imbalance and resource deficiencies.