Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Polymerase chain reaction.

H A Erlich1

  • 1Department of Human Genetics, Cetus Corporation, Emeryville, California 94608.

Journal of Clinical Immunology
|November 1, 1989
PubMed
Summary
This summary is machine-generated.

Polymerase Chain Reaction (PCR) offers in vitro DNA cloning, amplifying specific DNA fragments efficiently. This molecular biology technique is crucial for basic research and medical diagnostics.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Very high resolution single pass HLA genotyping using amplicon sequencing on the 454 next generation DNA sequencers: Comparison with Sanger sequencing.

Human immunology·2015
Same author

Next-generation sequencing can reveal in vitro-generated PCR crossover products: some artifactual sequences correspond to HLA alleles in the IMGT/HLA database.

Tissue antigens·2013
Same author

High throughput HLA genotyping using 454 sequencing and the Fluidigm Access Array™ System for simplified amplicon library preparation.

Tissue antigens·2013
Same author

16(th) IHIW : review of HLA typing by NGS.

International journal of immunogenetics·2013
Same author

Use of class I and class II HLA loci for predicting age at onset of type 1 diabetes in multiple populations.

Diabetologia·2012
Same author

Multi-locus HLA class I and II allele and haplotype associations with follicular lymphoma.

Tissue antigens·2012

Area of Science:

  • Molecular Biology
  • Biotechnology
  • Genetics

Background:

  • Recombinant DNA technology revolutionized molecular biology by enabling DNA replication through molecular cloning.
  • Traditional cloning involves inserting DNA into vectors for replication within host cells.

Purpose of the Study:

  • To highlight the impact and applications of Polymerase Chain Reaction (PCR) in molecular biology.
  • To position PCR as a key technology for both fundamental research and diagnostic applications.

Main Methods:

  • Polymerase Chain Reaction (PCR) as a method for in vitro DNA amplification and modification.
  • Comparison of PCR to traditional recombinant DNA techniques for DNA cloning.

Main Results:

Related Experiment Videos

  • PCR enables the generation of large quantities of specific DNA fragments from minimal template amounts.
  • PCR functions as an automated 'in vitro cloning' process, yielding DNA fragments of defined length and sequence.

    • Conclusions:

    • PCR significantly impacts basic molecular biology research and diagnostic applications.
    • PCR is vital for identifying and detecting medically important DNA sequences.