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Author Spotlight: Exploring the Role of FAM83A in Cervical Cancer
Published on: February 9, 2024
Roles of Long Non-Coding RNA CCAT2 in Cervical Cancer Cell Growth and Apoptosis
Le Wu1, Lixu Jin1, Wenmiao Zhang1
1Department of Obstetrics and Gynecology, First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, China (mainland).
Background:
This study aimed to analyze the potential function of lncRNA CCAT2 in cervical cancer cell proliferation and apoptosis.
Material/Methods:
Expression level of CCAT2 in cervical cancer cell lines (HeLa, CaSki, and SiHa) was detected by quantitative real-time PCR. CCAT2 knockdown was established by transfecting siRNA into human cervical cancer cells. Its effects on cell proliferation were studied using cell-counting kit-8 assay. The effect of CCAT2 on cervical cancer cells cycle and apoptosis was assessed by flow cytometry assay.
Results:
CCK8 assay showed that CCAT2 knockdown inhibited cell proliferation in HeLa, CaSki, and SiHa cells. The flow cytometry confirmed the results that knockdown of CCAT2 could induce cervical cancer cells cycle G1 phase arrestment and trigger the cells apoptosis.
Conclusions:
LncRNA CCAT2 promoted the proliferation and survival of cervical cancer cells.
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