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Related Experiment Video

Updated: Mar 24, 2026

Enumeration of Major Peripheral Blood Leukocyte Populations for Multicenter Clinical Trials Using a Whole Blood Phenotyping Assay
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Peripheral blood collection: the first step towards gene expression profiling.

Carmen Franken1,2, Sylvie Remy1,3, Nathalie Lambrechts1

  • 1a Unit Environmental Risk and Health, Flemish Institute for Technological Research (VITO NV) , Mol , Belgium ;

Biomarkers : Biochemical Indicators of Exposure, Response, and Susceptibility to Chemicals
|March 18, 2016
PubMed
Summary

Rapid handling of whole blood samples is vital for accurate gene expression analysis. Even short delays (under 30 minutes) significantly alter mRNA levels, impacting biomonitoring study results.

Keywords:
Bench timeIL-8RNA stabilitybiomarkerhuman blood sampling

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Area of Science:

  • Biomonitoring
  • Molecular Biology
  • Transcriptomics

Background:

  • Accurate gene expression analysis in human biomonitoring requires rapid handling and mRNA stabilization of whole blood samples.
  • Short bench times can compromise RNA yield, quality, and downstream gene expression profiling.

Purpose of the Study:

  • To evaluate the impact of short bench times (<30 minutes) on mRNA yield, quality, and gene expression.
  • To compare the efficacy of Tempus Blood RNA tubes and RNAlater Stabilization Reagent in preserving RNA integrity and gene expression profiles.

Main Methods:

  • Whole blood samples were collected and processed using Tempus Blood RNA tubes and RNAlater Stabilization Reagent.
  • RNA was extracted, and its yield and quality were assessed.
  • Microarray analysis was performed to evaluate gene expression profiles after short bench times.

Main Results:

  • Significant changes in the expression of 2356 genes were observed even with short bench times.
  • Affected gene networks included those mediated by NFκB, cancer pathways, and glucocorticoids.
  • Interleukin-8 (IL-8) showed particularly prominent changes in expression.

Conclusions:

  • Short bench times, even less than 30 minutes, significantly impact gene expression in whole blood.
  • Standardized blood collection protocols are essential to minimize pre-analytical variations in biomonitoring studies.
  • The choice of stabilization buffer may influence the extent of gene expression changes.