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Related Experiment Video

Updated: Mar 24, 2026

Combining Mitotic Cell Synchronization and High Resolution Confocal Microscopy to Study the Role of Multifunctional Cell Cycle Proteins During Mitosis

Combining Mitotic Cell Synchronization and High Resolution Confocal Microscopy to Study the Role of Multifunctional Cell Cycle Proteins During Mitosis

Published on: December 5, 2017

ABCE1 is essential for S phase progression in human cells.

Marina Toompuu¹, Kairi Kärblane¹, Pille Pata¹

¹a Department of Gene Technology , Tallinn University of Technology , Tallinn , Estonia.

Cell Cycle (Georgetown, Tex.)

|March 18, 2016

Summary

The essential protein ABCE1 is vital for human cell proliferation, impacting DNA synthesis and histone production. Its depletion halts cell growth by disrupting S phase progression.

Keywords:

ABCE1 DNA synthesis S phase eIF3 histone synthesis translation

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Published on: October 21, 2022

Area of Science:

Molecular Biology
Cell Biology
Biochemistry

Background:

ABCE1 is a conserved protein essential for eukaryotic and archaeal viability.
Initially identified as an RNase L inhibitor, ABCE1 is now known as a crucial translation factor.
The precise functions of ABCE1 remain largely unexplained.

Purpose of the Study:

To investigate the role of ABCE1 in human cell proliferation.
To explore the connection between ABCE1 function and protein translation.

Main Methods:

RNA interference (siRNA) was used to deplete ABCE1 in human cells.
Cell growth rates, DNA synthesis, and histone mRNA/protein levels were analyzed.
Association with eIF3 complex subunits was examined for transcript-specific translation analysis.

Main Results:

ABCE1 depletion led to reduced cell growth and S phase accumulation.
Inefficient DNA synthesis and decreased histone mRNA and protein levels were observed.
ABCE1's role in histone biosynthesis and DNA replication was inferred.

Conclusions:

ABCE1 is essential for normal S phase progression in human cells.
Beyond general translation, ABCE1 is implicated in histone biosynthesis and DNA replication.
ABCE1's direct role in transcript-specific translation via eIF3A was not consistently supported by the data.