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Advanced PCR-based molecular diagnosis of gastrointestinal infections: challenges and opportunities

Yuliya Zboromyrska1,2, Jordi Vila1,2

  • 1a Department of Clinical Microbiology, Biomedical Diagnostic Centre (CDB), Hospital Clínic, School of Medicine , University of Barcelona , Barcelona , Spain.

Expert Review of Molecular Diagnostics
|March 18, 2016
PubMed

Insights

Identifying the cause of acute diarrhea is crucial for effective treatment. This review examines molecular methods for detecting gastrointestinal pathogens, offering advantages over traditional techniques for diagnosing gastroenteritis.

Area of Science:

  • Microbiology
  • Infectious Diseases
  • Molecular Diagnostics

Background:

  • Gastrointestinal infections are common worldwide.
  • Accurate identification of enteropathogens (bacteria, viruses, protozoa) is vital for managing diarrheal diseases.
  • Conventional diagnostic methods like stool culture and microscopy have limitations in sensitivity, specificity, and timeliness.

Purpose of the Study:

  • To review and analyze currently available molecular methods for detecting various enteropathogens.
  • To evaluate the advantages and disadvantages of these molecular techniques for laboratory diagnosis of gastroenteritis.

Main Methods:

  • Literature review of molecular diagnostic techniques for enteropathogen detection.
  • Comparative analysis of molecular methods against conventional diagnostic approaches.

Main Results:

  • Molecular methods offer improved sensitivity and broader pathogen detection compared to traditional methods.
  • Different molecular platforms present varying benefits and drawbacks regarding cost, speed, and complexity.
  • Timely and accurate identification of pathogens is achievable with molecular diagnostics.

Conclusions:

  • Molecular methods represent a significant advancement in the laboratory diagnosis of gastroenteritis.
  • These techniques enhance the ability to identify a wide range of gastrointestinal pathogens, improving patient management.
  • Further research may focus on optimizing cost-effectiveness and accessibility of molecular diagnostics for infectious diarrhea.
Keywords:
DNA-based toolsGastroenteritisantigen testbacteriaculturediarrheaenteropathogensmicroscopic observationprotozoaviruses

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