Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Labeling DNA Probes

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Bis-Tetrazine Fluorogenic (Silicon)-Rhodamine Dyes for Live-Cell Labeling.

Journal of the American Chemical Society·2026

Same author

In preprints: building a resilient barrier - tissue mechanics control epithelial multilayering.

Development (Cambridge, England)·2026

Same author

3D epithelial cell topology tunes signaling range to promote precise patterning.

Proceedings of the National Academy of Sciences of the United States of America·2026

Same author

Direct RNA sequencing enables improved transcriptome assessment and tracking of RNA modifications for medical applications.

Nucleic acids research·2025

Same author

Lenacapavir-induced capsid damage uncovers HIV-1 genomes emanating from nuclear speckles.

The EMBO journal·2025

Same author

Differential conformational expansion of NUP98-HOXA9 oncoprotein from nanosized assemblies to macrophases.

Nature communications·2025

Search research articles

Related Experiment Video

Updated: Mar 24, 2026

Purification of Viral DNA for the Identification of Associated Viral and Cellular Proteins

Purification of Viral DNA for the Identification of Associated Viral and Cellular Proteins

Published on: August 31, 2017

Labeling of virus components for advanced, quantitative imaging analyses.

Volkan Sakin¹, Giulia Paci², Edward A Lemke²

¹Department of Infectious Diseases, Virology, University Hospital Heidelberg, Germany.

|March 19, 2016

Summary

Advanced microscopy enables single-particle virus imaging, revealing virus-cell interactions. Novel labeling strategies are crucial for maximizing the potential of these powerful virus imaging techniques.

Keywords:

amber suppression click labeling fluorescence human immunodeficiency virus imaging nanoscopy single-molecule techniques virus

More Related Videos

Single-Cell Multiplexed Fluorescence Imaging to Visualize Viral Nucleic Acids and Proteins and Monitor HIV, HTLV, HBV, HCV, Zika Virus, and Influenza Infection

Single-Cell Multiplexed Fluorescence Imaging to Visualize Viral Nucleic Acids and Proteins and Monitor HIV, HTLV, HBV, HCV, Zika Virus, and Influenza Infection

Published on: October 29, 2020

Method for Measurement of Viral Fusion Kinetics at the Single Particle Level

Method for Measurement of Viral Fusion Kinetics at the Single Particle Level

Published on: September 7, 2009

Related Experiment Videos

Last Updated: Mar 24, 2026

Purification of Viral DNA for the Identification of Associated Viral and Cellular Proteins

Purification of Viral DNA for the Identification of Associated Viral and Cellular Proteins

Published on: August 31, 2017

Single-Cell Multiplexed Fluorescence Imaging to Visualize Viral Nucleic Acids and Proteins and Monitor HIV, HTLV, HBV, HCV, Zika Virus, and Influenza Infection

Single-Cell Multiplexed Fluorescence Imaging to Visualize Viral Nucleic Acids and Proteins and Monitor HIV, HTLV, HBV, HCV, Zika Virus, and Influenza Infection

Published on: October 29, 2020

Method for Measurement of Viral Fusion Kinetics at the Single Particle Level

Method for Measurement of Viral Fusion Kinetics at the Single Particle Level

Published on: September 7, 2009

Area of Science:

Virology
Microscopy
Molecular Biology

Background:

Virus-cell interactions were traditionally studied using ensemble measurements.
Recent advancements allow for single-particle level investigations.

Purpose of the Study:

To compare labeling methods for single-particle virus imaging.
To discuss challenges and future directions in virus imaging.

Main Methods:

Advanced fluorescence microscopy techniques.
Single-molecule sensitivity and subdiffraction spatial resolution.
Novel labeling strategies tailored for viral constraints and microscopy requirements.

Main Results:

Demonstrated the capability of advanced microscopy for observing subviral details.
Provided examples of virological questions addressed using these techniques.
Highlighted the importance of optimized labeling for quantitative data acquisition.

Conclusions:

Single-particle virus imaging offers detailed quantitative insights into virus-cell interactions.
Strategic labeling is essential for leveraging advanced microscopy in virology.
Future developments hold significant potential for advancing virus imaging.