Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Integrated circuit-microfluidic biosensors for blood-based disease diagnostics-A review.

Analytica chimica acta·2026
Same author

Multi-task deep learning for sub-clinical screening of mood and sleep disturbances using physical activity biomarkers.

Journal of affective disorders·2026
Same author

Optimization of a microfluidic system for automated detection of Cholangiocarcinoma cells in bile.

Biosensors & bioelectronics·2025
Same author

Synthesis of Heparan Sulfate Hexadecasaccharides and Their Molecular Interaction with Mycobacterial Heparin-Binding Hemagglutinin for the Detection of <i>Mycobacterium tuberculosis</i>.

Journal of the American Chemical Society·2025
Same author

An integrated microfluidic system for automated extraction of <i>in vitro</i> transcribed mRNAs <i>via</i> probe-coated magnetic beads.

Lab on a chip·2025
Same author

An integrated microfluidic system with shear force control for an automatically modified mRNA display technique for screening high-specificity peptide probes.

Lab on a chip·2025
Same journal

Microfluidic rare cell analysis beyond counting: workflow design from enrichment to multi-omics.

Lab on a chip·2026
Same journal

A sperm racetrack to separate sperm by swim speed.

Lab on a chip·2026
Same journal

Controlled encapsulation and droplet size prediction in two-step microfluidic double emulsions.

Lab on a chip·2026
Same journal

A particulate blood-mimicking fluid with physiological biconcave geometry for microscale hemorheology.

Lab on a chip·2026
Same journal

Multicellular sensor arrays fabricated by capillary stamping for pattern-based odor discrimination.

Lab on a chip·2026
Same journal

A real-time microfluidic surveillance system for multiplex detection of heavy metal contamination in wastewater.

Lab on a chip·2026
See all related articles

Related Experiment Video

Updated: Mar 24, 2026

Automated Microfluidic Blood Lysis Protocol for Enrichment of Circulating Nucleated Cells
09:53

Automated Microfluidic Blood Lysis Protocol for Enrichment of Circulating Nucleated Cells

Published on: December 31, 2009

12.8K

Continuous nucleus extraction by optically-induced cell lysis on a batch-type microfluidic platform.

Shih-Hsuan Huang1, Lien-Yu Hung1, Gwo-Bin Lee2

  • 1Department of Power Mechanical Engineering, National Tsing Hua University, Hsinchu 30013, Republic of China. gwobin@pme.nthu.edu.tw.

Lab on a Chip
|March 19, 2016
PubMed
Summary
This summary is machine-generated.

This study introduces a novel microfluidic system for automated nucleus extraction using optically-induced cell lysis (OICL) and optically-induced dielectrophoresis (ODEP). This efficient method advances cell fusion and cloning technologies.

More Related Videos

Author Spotlight: Enhancing Drug Discovery - Development of Automated, Standardized Protocols for Nuclei Extraction from Frozen Tissues
07:12

Author Spotlight: Enhancing Drug Discovery - Development of Automated, Standardized Protocols for Nuclei Extraction from Frozen Tissues

Published on: July 28, 2023

5.3K
Rapid Subtractive Patterning of Live Cell Layers with a Microfluidic Probe
12:19

Rapid Subtractive Patterning of Live Cell Layers with a Microfluidic Probe

Published on: September 15, 2016

7.6K

Related Experiment Videos

Last Updated: Mar 24, 2026

Automated Microfluidic Blood Lysis Protocol for Enrichment of Circulating Nucleated Cells
09:53

Automated Microfluidic Blood Lysis Protocol for Enrichment of Circulating Nucleated Cells

Published on: December 31, 2009

12.8K
Author Spotlight: Enhancing Drug Discovery - Development of Automated, Standardized Protocols for Nuclei Extraction from Frozen Tissues
07:12

Author Spotlight: Enhancing Drug Discovery - Development of Automated, Standardized Protocols for Nuclei Extraction from Frozen Tissues

Published on: July 28, 2023

5.3K
Rapid Subtractive Patterning of Live Cell Layers with a Microfluidic Probe
12:19

Rapid Subtractive Patterning of Live Cell Layers with a Microfluidic Probe

Published on: September 15, 2016

7.6K

Area of Science:

  • Biotechnology
  • Microfluidics
  • Cell Biology

Background:

  • Nucleus extraction is critical for disease diagnosis, genetic replication, and animal cloning.
  • Current nucleus extraction methods are inefficient and labor-intensive.

Purpose of the Study:

  • To develop an automated, microfluidics-based system for efficient nucleus extraction.
  • To integrate optically-induced cell lysis (OICL) and optically-induced dielectrophoresis (ODEP) for enhanced nucleus isolation.

Main Methods:

  • A microfluidic chip integrating OICL and ODEP modules was designed for automated cell processing.
  • The system automates cell focusing, transport, nucleus release via OICL, nucleus isolation via ODEP, and collection.
  • Key efficiencies measured include cell membrane lysis (78.04 ± 5.70%) and ODEP nucleus separation (80.90 ± 5.98%).

Main Results:

  • The integrated microfluidic system achieved an overall nucleus extraction efficiency of 58.21 ± 2.21%.
  • The OICL and ODEP modules demonstrated high selectivity and efficiency in nucleus extraction.
  • The system eliminates the need for highly trained personnel and expensive equipment.

Conclusions:

  • The developed microfluidics-based system successfully automates nucleus extraction.
  • This integrated platform shows significant promise for applications in cell fusion, genetic replication, and animal cloning.