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Related Concept Videos

Reporter Genes02:11

Reporter Genes

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Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
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Radionuclide-fluorescence Reporter Gene Imaging to Track Tumor Progression in Rodent Tumor Models
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Multimodal molecular imaging system for pathway-specific reporter gene expression.

Marco Rossi1, Luisa Massai1, Daniela Diamanti1

  • 1Siena Biotech Medicine Research Centre, Siena, Italy.

European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences
|March 19, 2016
PubMed
Summary

This study combined Optical Imaging (OI) and Magnetic Resonance Imaging (MRI) for preclinical research. The integrated system successfully monitored Wnt pathway response to Lithium Chloride (LiCl) treatment in glioblastoma models.

Keywords:
BLIMRIMolecular ImagingMultimodal imagingReporter gene

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Area of Science:

  • Biomedical Research
  • Molecular Imaging
  • Preclinical Studies

Background:

  • Optical Imaging (OI) and Magnetic Resonance Imaging (MRI) are crucial preclinical tools, often used separately.
  • Combining OI and MRI offers enhanced capabilities for translational research.

Purpose of the Study:

  • To develop a combined OI and MRI system for monitoring molecular responses in vivo.
  • To assess the Wnt pathway response to Lithium Chloride (LiCl) treatment in glioblastoma models.

Main Methods:

  • A lentiviral vector was developed to co-express human ferritin heavy chain protein (hFTH) for MRI and firefly luciferase (FLuc) for OI.
  • Glioblastoma cells with the construct were transplanted into mice, followed by serial in vivo OI and 7 Tesla MRI.
  • Ex vivo analysis utilized constitutive fluorescence protein expression.

Main Results:

  • Lithium Chloride (LiCl) administration significantly increased luminescence signal (OI).
  • LiCl treatment resulted in lower T2 values (MRI), indicating treatment efficacy.
  • Combined OI and MRI provided complementary in vivo data.

Conclusions:

  • OI and MRI can be effectively integrated into a single in vivo preclinical experiment.
  • This dual-modality approach offers a proof-of-concept for drug discovery and development.
  • The developed system enhances molecular imaging capabilities in translational research.