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Related Experiment Videos

Replication control and cellular life span.

S M Jazwinski1, N K Egilmez, J B Chen

  • 1Department of Biochemistry and Molecular Biology, Louisiana State University Medical Center, New Orleans 70112.

Experimental Gerontology
|January 1, 1989
PubMed
Summary
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Researchers identified a heat-sensitive protein kinase in yeast that may control DNA replication initiation. They also found that aging yeast cells exhibit a dominant, cytoplasmically controlled senescence, influencing cell cycle coordination.

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Genetics

Background:

  • Cell proliferation requires precise control of cell cycle progression and coordination between successive cycles.
  • The G1/S boundary is a critical checkpoint for initiating DNA replication.
  • Cellular aging in yeast (Saccharomyces cerevisiae) involves a finite reproductive lifespan and changes in generation time.

Purpose of the Study:

  • To investigate the molecular mechanisms controlling the G1/S boundary traversal in the cell cycle.
  • To explore the relationship between cell cycle regulation and cellular aging in yeast.
  • To identify factors contributing to yeast senescence and their role in coordinating successive cell cycles.

Main Methods:

  • Isolation and characterization of a protein kinase activity associated with the DNA-replicative complex in yeast.

Related Experiment Videos

  • Analysis of heat sensitivity of protein kinase activity in cdc7 mutant yeast cells.
  • Study of yeast cell aging, including generation time measurements and analysis of mother-daughter cell cycle control.
  • Cloning of genes differentially expressed during the yeast lifespan to identify potential senescence factors.

    • Main Results:

    • A heat-sensitive protein kinase activity was identified in the DNA-replicative complex, preferentially phosphorylating a 48-kDa polypeptide.
    • This kinase activity was impaired in cdc7 mutants, which arrest at the G1/S boundary.
    • Aging yeast cells showed lengthened generation times, with a transient dominant control of the mother cell cycle over the daughter cell cycle.
    • Evidence suggests a diffusible cytoplasmic molecule(s) underlies the dominant senescence phenotype in yeast.

    Conclusions:

    • Phosphorylation of replication machinery components likely plays a role in controlling DNA replication initiation during the cell cycle.
    • Cellular aging in yeast is characterized by a dominant, cytoplasmically determined senescence phenotype.
    • The Cell Spiral model is proposed to describe the interplay between the cell cycle and cellular aging.