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A green analytical method for rapid determination of pectin degree of esterification using micro sequential injection
Mahsa Naghshineh1, Jan Larsen2, Constantinos Georgiou3
1Department of Food Science, Faculty of Science, University of Copenhagen, Rolighedsvej 30, DK-1958 Frederiksberg C, Denmark; Chemistry Laboratory, Agricultural University of Athens, Iera Odos 75, 11855 Athens, Greece.
Abstract:
A novel method for automated determination pectin degree of esterification (DE) using micro sequential injection lab-on-valve (μSI-LOV) system is developed. A face-centered central composite response surface methodology (RSM) was used to optimise system parameters. A calibration graph for determination of non-esterified galacturonic acid (GalA) content in pectin solutions with linear range of 0.08-0.34% (w/v) and the limit of detection (LOD) of 0.057% (w/v) under optimal condition was achieved. The difference between concentrations (w/v, %) of total GalA and non-esterified GalA was applied to estimate DE (%) of pectin samples. Results indicated a good agreement (tstat
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