Related Experiment Video
Updated: Mar 24, 2026
Optimized Interferon-gamma ELISpot Assay to Measure T Cell Responses in the Guinea Pig Model after Vaccination
Published on: January 20, 2019
Adapted Lethality: What We Can Learn from Guinea Pig-Adapted Ebola Virus Infection Model
S V Cheresiz1, E A Semenova1, A A Chepurnov2
1Department of Medicine, Novosibirsk State University, Pirogova Street 2, 630090 Novosibirsk-90, Russia; Institute of Internal and Preventive Medicine, Bogatkova Street 175/1, 630089 Novosibirsk-89, Russia.
Abstract:
Establishment of small animal models of Ebola virus (EBOV) infection is important both for the study of genetic determinants involved in the complex pathology of EBOV disease and for the preliminary screening of antivirals, production of therapeutic heterologic immunoglobulins, and experimental vaccine development. Since the wild-type EBOV is avirulent in rodents, the adaptation series of passages in these animals are required for the virulence/lethality to emerge in these models. Here, we provide an overview of our several adaptation series in guinea pigs, which resulted in the establishment of guinea pig-adapted EBOV (GPA-EBOV) variants different in their characteristics, while uniformly lethal for the infected animals, and compare the virologic, genetic, pathomorphologic, and immunologic findings with those obtained in the adaptation experiments of the other research groups.
