[Vectors for constructing representative genome libraries]

The general methodology for constructing genomic libraries of different types of vectors is discussed. Various ways of selection against non-recombinant molecules in generated libraries are considered. The general features of well-known vectors (lambda EMBL3, lambda EMBL4) and of new ones (lambda Ch40, lambda SK5, lambda FIX, pWE and other) are presented. A special attention is paid to vectors lambda SK9 and SK18 that have the features of lambda and M13 phages and of plasmids (diphasmids). Data on phasmids (lambda pMYF131 and lambda pSL51) and hyphages MC18 and MC19 are also presented.